SAFETY TESTING OF HAIR DYE PREPARATIONS 753 sex) weighing 2 to 3 kg. were prepared 24 hours prior to start of applica- tion. The entire dorsal surface was closely clipped by means of an electric clipper, removing as much hair as possible while avoiding nicking or abrading of the skin. The P.P.D.-R. dye-peroxide mixtures were prepared in accordance with the label instructions and applied five days a week for six hours to the body surface under plastic sleeves, following which the sleeves were removed. The excess was wiped off without shampooing. Skin scores were read daily. The physiological status of the rabbits was determined prior to and after the last applica- TABLE IV Body Weights i-Hour Contact with P.P.D.-R. Dye -3- H202 Dose Factor ----- Days No. of Rabbits 0 21 Net Gain 0 5M 5F lx 5M 5F 2x 5M 5F 23-2.6 25-3.7 24-2.7 24-2.9 22-2.8 23-2.9 2.4-3 2.7-3 2.8-3 2.7-3 2.5-3 2.7-3 0 •1.1 9 •0.9 0 0.3-0.6 3 0.5-0.7 0 0.3-1.1 5 0.3-0.7 TABLE V Body Weights--Prolonged Contactwith P.P.D.-R. Dye+ H202 Multiple of Days , Human Dose s 0 21 Net Gain 0 1.9-2.1 2.3-2.6 0.2-0.7 ix 1.7-2.3 1.•2.5 --0.5-0.6 3x 2.•2.6 2.4-2.8 --0.2-0.4 10x 1.•2.6 2.•2.7 b 0.1-0.2 Three rabbits per treatment group (males). One animal died in this group. tion. Blood and urine examinations were made as above described. The animals were examined daily, and unusual signs were recorded. They were sacrificed by over-barbitalization at the end of the 21st day after 15 test exposures or at 35 days, examined grossly at necropsy, and tissues and organs examined microscopically with particular emphasis being placed on the skin and adnexal areas. From the observations made upon the differential pattern of ery- thema and injection of the vascular network of the dermal surface, it was ascertained that the gross scoring of the epidermal surface failed to reflect apparent differences between products or graded concentra-

754 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS '7'7