240 JOURNAL OF COSMETIC SCIENCE DISCUSSION Antioxidants in skin care products have been found to be effective protectants against free radical-mediated oxidative damage in skin. Also, due to the rise in photodamage in an aging population, the topical application of cosmetic products that contain antioxi- dants has become an important area of research in skin care products. Thus, the need to measure the activity of antioxidants in a finished product is of critical importance. In this report we describe a novel and simple technique to quickly assess the relative antioxidant potential of whole product formulations. Additionally, it can be utilized for stability studies and, since the chromogen develops in the visible region, perhaps even a quali- tative result can be obtained by workers in the field who lack spectrophotometers but need to assay the antioxidant activity of a product. REFERENCES (1) B. A. Gilchrest, Skin and Aging Processes (CRC Press, Boca Raton, FL, 1989), pp. 97-116. (2) A. V. Benedetto, The environment and skin aging, Clin. Dermatol., 16, 129-139 (1998). (3) J. Fuchs and L. Packer, Eds., Oxidative Stress in Dermatology (Marcel Dekker, New York, 1993). (4) E. Pelle, D. Maes, G.A. Padulo, E-K. Kim, and W. P. Smith, An in vitro model to test relative antioxidant potential: Ultraviolet-induced lipid peroxidation in liposomes, Arch. Blochem. Biophys., 283, 234-24O (1990). (5) E. Pelle, N. Muizzuddin, T. Mammone, K. Marenus, and D. Maes, Protection against endogenous and UVB-induced oxidative damage in stratum corneum lipids by an antioxidant-containing cosmetic formulation, Photodermatol. Photoimm•nol. Photoreed., 15, 115-119 (1999).

j. Cosmet. Sd., 53, 241-242 (July/August 2002) Abstracts Journal of the Society of Cosmetic Chemists Japan Vol. 35, No. 3, 2001' Characterization of Eluted Proteins from Hair Fiber under Permanent Waving or Bleaching* Takafumi Inoue, Mayumi Ito, Kenji Kizawa Basic Research Laboratory, Kanebo Ltd.** Protein is the most abundant component (65-95%) of hair, and has been targeted as an important subject for hair science research. Chemical treatments, such as permanent waving or bleaching, are widely known to be a major cause of hair damage. However, the constituent proteins in the effluent from these treatments have not been characterized in detail. In this study, we performed Tricine-SDS-polyacrylamide gel electrophoresis for detailed analyses of the proteins eluted under various chemical treatment conditions. Effluents from permanent waving or bleaching showed similar electrophoretic profiles, and both types contained a major protein band of approximately 7kDa. Through immunoblot analyses, this protein band was identified as ubiquitin, a ubiquitously distributed protein that mediates non-lysosomal protein degradation in eukaryotic cells. Comparative analyses of the ubiquitin-signal-intensities revealed that natural hair extracts derived from distal parts contained a lower ubiquitin content than those from proximal parts. These results suggested that ubiquitin was released during the course of natural occurring hair damage. Therefore, we speculated that the major components lost from hair were likely to be soluble proteins that were neither keratin intermediate filament proteins nor keratin associated proteins. camera system. The subjects whose sag scores increase followed by age were photographed by moire 3- dimension camera. When an arbitrary 3-dimensional point (a, b, c) was chosen on the facial skin under a nose, a curve of facial outline was obtained as a 2- dimensional curve on xz-plane (y=b) that includes the point (a, b, c). This curve includes a morphological structure of nasolabial groove. Then we determined their differential curves and their maximum peaks around nasolabial groove to analyze changes of facial morphological structure, followed by a confirmation of the high correlation between the maximum peak value of differential curve and age or sag score. Furthermore, we compensated the maximum peak value of differential curve on the basis of its differential value of the central side around nasolabial groove in order to tandardize the individual topographic differences. As a result, the strong correlation between these peaks and age or sag score was revealed. These results suggested that the nasolabial groove can represent the facial sag and that evaluation of the nasolabial groove is a very useful way. Moreover, because of its high ability of quantification without any proper experiences, this method is very useful for sag evaluation. Evaluations of Appearance of the Foundation Applied Skin and Some Ideas for Improvements of Long Lasting Performance* Tsuyoshi Ogihara Product Research Laboratory, KOSE Corporation** The Method of Evaluation for Facial Sag by the Analysis of Topography around Nasolabial Groove* Naoko Endo, Takafumi Nishijima, Tsutomu Fujimura, Shigeru Moriwaki, Yoshinori Takema Biological Science Laboratories, Kao Corporation** Facial sag becomes tangible accompanied with aging. We have previously reported a usefulness of photonumeric scale for facial sag evaluation. In this paper, we investigated the quantitative method to evaluate facial sag precisely using a moire 3-dimension 'Long lasting performance' is one of the important qualities for make-upcosmetics. The long lasting performance also has been understood as a total quality of the make-up appearances last long, has been evaluated mainly using the sensory analysis. In this report, for some evaluation ways of the make-up appearances and the long lasting performances, on the make-up foundation case, will be introduced to sensory analysis, an optical measurement and some substitute properties. Moreover, it will be introduced to the improvement technologies for aspects of the application techniques and the applied ingredient properties. * These abstracts appear exactly as they were originally published. They have not been edited by the Journal of Cosmetic Science. 241