332 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Of the younger female rats, three months old, receiving 0.1 mi. daily in the head region (Group 10) only one showed any sign of hair loss. This one developed a small denuded area of less than 0.4 cm. across after five days of treatment. In the flank area, however, neither one nor three injections (Groups 11 and 12) per day caused any loss of hair. The older females over seven months of age did acquire furless areas fol- lowing ten days of injections either in the head area after single injections (Group 13) or in the flank after single or triple injections (Groups 14 and 15). Ulcerated areas with scab formation appeared on the flanks of two rats from each of the latter groups. TABLE 1.--INJECTIONS OF METHOXAMINE HYDROCHLORIDE IN SALINE SOLUTION Days Injec- of tions Age Wt., Dose, In)ec- per Results at Group Sex mos. gm. mi. Site t•on Day Injection Sites 1 M 52/4 350 0.25 2 M 51/4 350 0.25 3 M 5ø/4 350 0.25 4 M 53/4 350 0.25 5 M 61/4 350 0.25 6 M 61/4 350 7 M 31/4 350 8 M 31/4 350 9 M 41/s 350 10 F 3 2OO 11 F 3 200 12 F 3 200 13 F 71/4 230 14 F 73/4 230 0.25 01 01 01 01 0.1 0.1 0.1 0.1 15 F 7a/4 230 0.1 Hd 4 1 HA* 1-2 cm. across in 5 rats Hd 4 3 HA 1-2 cm. in 5 rats FI 5 3 HA 2-4 cm. in 5 rats FI 5 1 Ha 0.5 cm. in 1 rat Thin Ft in 4 rats Hd 10 1 HA 0.5-1 cm. in 5 rats Sc. $ on 1 rat F1 10 1 HA 2 cm. in 5 rats Hd 10 1 HA 0.5 cm. in 4 rats F1 10 1 HA 0.5-1 cm. in 2 rats Thin F 0,5-1 cm. in 3 rats FI 10 3 HA 1-1.5 cm. in 2 rats Thin F 0.5 cm. in 1 rat Sc. on 2 rats Hd 5 1 HA 0.5 cm. in 1 rat FI 5 1 No effects FI 5 3 No effects Hd ] 0 l HA 0.5-1 cm. in 5 rats F1 10 1 HA 1-3 cm. in 4 rats ThinF l X 2cm. in 1 rat Sc. on 2 rats F1 10 3 HA 1-2 cm. in 4 rats ThinF1 X lcm. inlrat Sc. on 2 rats * HA--Hairless areas, cm. across. t Thin F--Thinning fi•r, cm across. $ Sc.--Small scabs at sites of injections. Following the daily administration of estradiol (100 y/kg.) to the male rats and of methyl testosterone (1000 y/kg.) to the females for a week methoxamine hydrochloride was injected for a four-day period. Hairless areas appeared about injection sites in the males following single daily injections in the scalp (Group 16) and also in the flank following daily triple injections (Group 17) (see Table 2). Two animals in the former and one in the latter groups developed small scabs at the treatment sites. Some loss of hair appeared in the head region of three of the females

ALOPECIA AND CUTANEOUS ULCERATION IN RATS 333 given the male sex hormone (Group 18) and one had a similar effect from treatment on the side (Group 19). Two others in this group had thinning of fur about the injection sites. These results indicate that such short treatment with sex hormones do not appreciably alter the usual results with methoxamine hydrochloride although they suggest that the male hormone may possibly increase the activity of the drug in females. TABLE 2.--INJECTIONS OF METHOXAMINE HYDROCHLORIDE INTO RATS RECEIVING ESTRADIOL OR METHYL TESTOSTERONE -- Days lnjec- of tions Results at Age, Wt., Dose, Injec- per Injection Group Sex mos. gm. ml. Site tion Day Sites 16 M 8 350 0.25 Hd 4 1 HA* 2 cm. in 5 rats Sc.•- on 2 rats 17 M 8 350 0.25 F1 4 3 HA 1-3 cm. in 5 rats Sc. on 1 rat 18 F 41/4 200 0.1 Hd 4 1 Thin F{ 1 X 1 cm. in 3 rats 19 F 41/4 200 0.1 F1 4 3 HA 1 X 2 cm. in 1 rat Thin F in 2 rats * HA Hairless areas, cm. across. •- Sc.--Small scabs at sites of injections. :[ Thin F--Thinning fur, cm. across. TABLE 3.--INJECTIONS OF MF•THOXAMINE HYDROCHI,ORIDE SUSPENDED IN PEANUT OIL Days Injec- of tions Results at Age, Wt., Dose, Injec- per Injection Group Sex mos. gm. ml. Site tion Day Sites 20 M 61/2 350 0.25 Hd 9 1 HA* 0.5-1 cm. in 5 rats (Oil depots re- mained for weeks) 21 F 101/,2 230 0.25 Hd 9 1 HA 1-1.5 cm. in 4 rats Thin Ft 1 X 1.5 cm. in 1 rat (Oil depots remained for weeks) 22 F 101/.2 230 0.25 FI 7 1 Ulcerations 2-3 cm. on 5 rats * HA--Hairless areas, cm. across. • Thin F--Thinning fur, cm. across.