ABSTRACTS 319 BL or DF to lotion, milky lotion, body soap or shampoo and diluting with methanol, the extract was analyzed by HPLC using a Unisil Q C18 column (4.6 mm i.d. x 150mm), a mixture of acetonitrile and 50 mM phosphate buffer (pH 3.5) (13:7) for BL or (1:1) for DF as the mobile phase and the detection wavelength of 306 nm for BL and either 227 nm or 286 nm for DF, respectively. In the experiment curves for BL and for DF ranged from 1 to 10 g g/mL and from 0.1 to 1.2 or 1 to 12 g g/mL. There was good correlation between the concentration and the peak area of BL or DF. BL and DF added to lotion, milky lotion, body soap or shampoo-except for milky lotion D-were completely determined by HPLC. In the case of milky lotion D, an ingredient in that lotion interrupted the peak of DF on the HPLC chromatogram. So a pre-screening method with a short silica gel column was employed and the DF in milky lotion D was satisfactorily determined by HPLC. Recent Progress in Skin Bioengineering Techniques and Its Application to Evaluation of Skin Care Cosmetics Motoji Takahashi Shiseido Research Center (Shin-Yokohama) Recently there has been increasing concern about the properties and efficacy of cosmetics because the government regulation of the effects of cosmetic has been eased and the publicizing of the skin care effects of foundations or lipsticks has been officially permitted since April 2001. On the other hand, bioengineering techniques closely related to the substantiation of cosmetics have been well-developed and nowadays the internal structure of human skin and cutaneous cells can be studied non-invasively using the methods. In this paper I describe a few such techniques, which are in vivo measurement of the microtopography of human skin (texture and wrinkles), in vivo confocal lazer microscopy to study depigmentation, optical coherence tomography (OCT) for stratum comeum thickness and epidermal - dermal undulation measurement, and in vivo confocal Raman microspectrogcopy for measurement of water distribution in strattlm corneum. The possibility of application to efficacy testing of cosmetics is also mentioned. Sonochemical Effects due to Cavitation Generated by an Ultrasonic Wave Beauty Care Device Yoshihiro Kojima, Kazunori Futamura, Shinobu Koda Research Center for Advanced Waste and Emission Management, Nagoya University Department of Molecular Design and Engineering Graduate School of Engineering, Nagoya University It is not clear whether or not commercial ultrasonic wave devices including beauty care devices, toothbrushes, and washing machines, produce cavitation and sonochemical effects. In our work, the cavitation and SOhochemical effects of a commercial ultrasonic wave beauty care device in water and in an aqueous solution of potassium iodide exposed to air were investigated. Nitric and nitrous ions were formed in distilled water under sonication, and their concentration increased with increasing sonication time. When ultrasound was irradiated into the aqueous solution of potassium iodide, the iodide ion was oxidized to a tri iodide ion. This was due to the hydrogen peroxide produced in the solution under sonication. Our results indicated that sonication from ultrasonic wave beauty care device produces cavitation and sonochemical effects in water.