DETERMINATION OF 13 COMPONENTS IN OXIDATIVE HAIR DYES 329 2,5-Diaminotoluene 125 105.3 84.2 2.22 112.5 90.1 2.32 250 217.3 86.9 1.08 218.5 87.4 1.53 1250 1185 94.8 2.04 1198 95.7 2.66 2500 2540 101.6 1.42 2533 101.3 1.04 3,4-Diaminetoluene 125 117.5 93.9 1.87 117.8 94.2 1.77 250 244.0 97.6 2.64 245.0 98.0 2.17 1250 1153 92.3 2.40 1248 99.9 2.23 2500 2290 91.6 1.63 2525 101.0 0.80 N,N-diethyl-p- phenylenediamine 250 238.0 95.2 1.98 247.5 99.0 1.91 500 477.5 95.6 2.54 490.0 97.8 2.32 2500 2550 102.0 0.90 2253 90.1 1.26 a Hair dye A contained 536.6 mg/kg resorcinol. b Hair dye B contained 3528 mg/kg m-aminophenol, 6618 mg/kg p-aminophenol, and 8727 mg/kg p-phenylenediamine. Table II Continued Compound Added (mg/kg) Hair dye Aa Hair dye Bb Found (mg/kg) Recovery (%) C.V. (%) Found (mg/kg) Recovery (%) C.V. (%) ACCURACY AND RECOVERY The results of the standard recovery test (Table II) showed that the average recoveries were in the range of 81.7–102.0% with four addition levels of two original commercial samples, and the RSDs (relative standard deviation) were about 0.9–3.5%. The precision data obtained from the evaluation of the results of a collaborative trial involving fi ve laborato- ries and three replicates with hair dye A (containing 536.6 mg/kg resorcinol) and hair dye B (containing 3528 mg/kg m-aminophenol, 6618 mg/kg p-aminophenol, and 8727 mg/kg p-phenylenediamine) were shown in Table III. CONCLUSIONS A sensitive and accurate analytical method for the determination of 13 dye intermediates in commercial hair dyes was developed. The introduction of sodium dithionite as a reductant Table III The Repeatability (r) and Reproducibility (R) of This Method (mg/kg) (20) Sample Compound ma Srb SRc rd Re Hair dye A Resorcinol 553 10 12 29 34 Hair dye B m-Aminophenol 3525 66 68 183 189 p-Aminophenol 6631 60 90 167 252 p-Phenylenediamine 8636 78 78 217 217 a m is the general average. b Sr is the repeatability standard deviation. c SR is the reproducibility standard deviation. d r is calculated according to ISO 5725-2:1994. e R is calculated according to ISO 5725-2:1994.

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