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J. Cosmet. Sci., 65, 285–298 (September/October 2014) 285 Anti-skin-aging benefi ts of exopolymers from Aureobasidium pullulans SM2001 KYUNG HU KIM, SOO JIN PARK, JI EUN LEE, YOUNG JOON LEE, CHANG HYUN SONG, SEONG HUN CHOI, SAE KWANG KU, and SU JIN KANG Department of Histology and Anatomy, College of Korean Medicine, (K.H.K., S.J.P., J.E.L., C.H.S., S.H.C., S.K.K.), The Medical Research Center for Globalization of Herbal Medicine, (S.J.P., J.E.L., Y.J.L., C.H.S., S.H.C., S.K.K., S.J.K.), and Department of Preventive Medicine, College of Korean Medicine, Deagu Haany University, Gyeongsan, Republic of Korea (Y.J.L., S.J.K.). Accepted for publication July 6, 2014 Synopsis Background: There have been many attempts to search for affordable and effective functional cosmetic ingredients, especially from natural sources. Objectives: As research into developing a functional cos- metic ingredient, we investigated whether exopolymers from Aureobasidium pullulans SM2001 (E-AP- SM2001) exert antioxidant, antiwrinkle, whitening, and skin moisturizing effects. Methods: Antioxidant effects of E-AP-SM2001 were determined by measuring free radical scavenging capacity and superoxide dismutase (SOD)-like activity. Antiwrinkle effects were assessed through the inhibition of hyaluroni- dase, elastase, collagenase, and matrix metalloproteinase (MMP)-1. Whitening effects were measured by tyrosinase inhibition assay, and by melanin formation test in B16/F10 melanoma cells. Skin moisturiz- ing effects were detected by mouse skin water content test. Results: E-AP-SM2001 showed potent DPPH radical scavenging activity and SOD-like effects. Additionally, hyaluronidase, elastase, collage- nase, and MMP-1 activities were signifi cantly inhibited by E-AP-SM2001. We also observed that E-AP- SM2001 effectively reduced melanin production by B16/F10 melanoma cells and mushroom tyrosinase activities. Furthermore, signifi cant increases in skin water content were detected in E-AP-SM2001- treated mouse skin, as compared with vehicle-treated control skin. Notably, a mask pack containing E- AP-SM2001 showed a twofold more extensive moisturizing effect compared with one containing Saccharomycopsis ferment fi ltrate. Conclusions: Our results suggest that E-AP-SM2001 has adequate an- tiaging, antiwrinkle, and whitening benefi ts and skin moisturizing effect. These effects involve reducing hyaluronidase, elastase, collagenase, and MMP-1 activities, as well as inhibition of melanin production and tyrosinase activities. Therefore, the antioxidant E-AP-SM2001 may serve as a predictable functional ingredient. Address all correspondence to Sae Kwang Ku at gucci200@hanmail.net and Su Jin Kang at vegonia1@ hanmail.net. Kyung Hu Kim, Soo Jin Park, and Ji Eun Lee contributed equally to this work.