STAINING CATIONICS ON KERATIN 213 S•CI C-10-2 SAC & C. ALC. C-10-2 S XC/SI, S SAC & C. ALC. SLS -- SAC CREME RINSE SAC CREME RINSE C - 10 - 2 SLS SAC Figure 6. Treatment of wool swatches with a simple cationic vs. formulated conditioners and washing these with deceth vs. lauryl sulfate and staining with Red-80. Table II Radiotracer Analysis Showing Quantities of Anionic and Cationic Moieties Bound to Keratin After Treatment With a Conditioner Product # and Washing With Anionic Detergents mg Ionic moiety/gm Dry keratin Treatment Anion Cation Total bound Conditioner/ALS 4.0* 1.7 5.7* Conditioner/SDES 2.0 1.9 3.9 # Keratin contained 4.5 mg SAC/gm dry keratin after conditioner treatment, prior to washing. * Significantly different from corresponding value at or beyond the 95% level.

214 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS nomenon of buildup on hair, where the anionic detergent of shampoo actually builds up on hair with the cationic conditioner by forming association complexes. Thus, the modified dye-staining procedure is a useful screening tool to help study the removal of cationic conditioners from hair. However, because of the complexity of these reactions, the dye-staining test should be used with other techniques to assist in inter- preting the results.. REFERENCES (1) G. V. Scott, C. R. Robbins, and J. D. Barnhurst, Sorption of quaternary ammonium surfactants by human hair, J. Soc. Cosmet. Chem. 20, 135-152 (1969). (2) R. J. Crawford and C. R. Robbins, A replacement for Rubine dye for detecting cationics on keratin, J. Soc. Cosmet. Chem., 31, 273-278 (1980). (3) Purchased from Test Fabric, Inc., Middlesex, N.J. (4) Purchased from A. Klugman, New York, N.Y. (5) Sirius Red F3BA New, C.I. No. 35780, Mobay Chemical Corp., Rock Hill, S.C. Equivalent products are offered by Sandoz Colors & Chemicals, E. Hanover, N.J., and by American Color & Chemical Corp., Charlotte, N.C. (6) C. R. Robbins, C. Reich, and J. Clarke, submitted for publication. (7) R. L. Sneath. Presented at the 5th International Hair Science Symposium in Constance, W. Ger- many, November 1986. APPENDIX Summary of the Staining Reaction Results After Treatment With SAC or CTAC and Washing With Anionic Detergent and Then Staining With Red-80 Dye Anionic detergent Staining results Sodium octyl sulfate Sodium decyl sulfate Sodium lauryl sulfate Ammonium lauryl sulfate Sodium tetradecyl sulfate Sodium deceth-2 sulfate Sodium dodeceth-1 sulfate Sodium dodeceth-2 sulfate Sodium dodeceth-3 sulfate Sodium dodeceth-5 sulfate Sodium dodeceth-7 sulfate Sodium dodeceth-12 sulfate Alpha olefin sulfonate (C10) Alpha olefin sulfonate (C12) Alpha olefin sulfonate (C 14) Alpha olefin sulfonate (C16) Stains Stains No stain No stain No stain Stains No stain No stain No stain No stain Stains Stains Stains Stains No stain No stain