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J. Cosmet. Sci., 65, 217–224 (July/August 2014) 217 Internal structure changes of eyelash induced by eye makeup KEN-ICHI FUKAMI, TAKAFUMI INOUE, TOMOMITSU KAWAI, MUNEKI SAKATA, MASAHIRO NAGANO, KOUJI TAKEHARA, AKIHISA TAKEUCHI, KENTARO UESUGI, and YOSHIO SUZUKI, Skincare Research Laboratory (K.F., T.K., K.T.), Innovative Beauty Science Laboratory (T.I.), Make-up Research Laboratory (M.S., M.N.), Kanebo Cosmetics Inc., Kotobuki-cho 5-3-28, 250-0002, Japan, and Japan Synchrotron Radiation Research Institute, Koto 1-1-1, Hyogo 679-5198 (A.T., K.U., Y.S.), Japan. Accepted for publication May 27, 2014. Synopsis To investigate how eye makeup affects eyelash structure, internal structure of eyelashes were observed with a scanning X-ray microscopic tomography system using synchrotron radiation light source. Eyelash samples were obtained from 36 Japanese women aged 20–70 years and whose use of eye makeup differed. Recon- structed cross-sectional images showed that the structure of the eyelash closely resembled that of scalp hair. The eyelash structure is changed by use of eye makeup. There was a positive correlation between the fre- quency of mascara use and the degree of cracking in cuticle. The positive correlation was also found between the frequency of mascara use and the porosity of the cortex. By contrast, the use of eyelash curler did not affect the eyelash structure with statistical signifi cance. INTRODUCTION Whether eye makeup induces characteristic changes in the structure of eyelashes is of interest. The growth cycle of the eyelash is approximately 90 days (1), which is shorter than that of scalp hair of two to six years (2). Therefore, there would appear to be less opportunity for eyelashes to be damaged than scalp hair. It has been re- ported that characteristics of eyelashes are not infl uenced by eye makeup use (3). However, the makeup treatments that are applied to the eyelash differ from those that are applied to the scalp hairs, e.g., mascara, eyelash curler, false eyelashes, and eyelash extensions. Eyelashes might be damaged by these treatments. In this study, we investigated the effects of mascara and eyelash curler use on eyelash internal structure. Address all correspondence to Takafumi Inoue at inoue.takafumi@kanebocos.co.jp.