144 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS TABLE V--SUBACUTE DERMAL STUDIES IN RABBITS TREATED WlTI-I SLS--LEUKO½¾TE DATA Rabbit Dose,* No. & mg./kg. Sex Leuko- cyte Skin Count, Treat- Period, X10a/ ,------Differential Count, %{----• mentt days mm.a Polys Lymph Eos Mono Bas 0 19M 20 M 21 F 6 1M 2M 3M 4F 5 F 6F 60 7 M 8M 9M 10 F 11F 12F 150 13 M 14 M 15M 16M 17 F 18F A 0 6.8 16 84 0 0 0 21 8.7 21 75 0 3 1 I 0 18.0 8 91 1 0 0 21 4.9 24 74 0 0 2 90 11.9 22 76 0 0 2 I 0 7.8 11 83 1 1 4 21 7.1 19 79 0 2 0 90 9.9 28 69 0 0 3 A 0 14.2 7 88 1 2 2 21 6.0 23 75 0 1 1 I 0 5.6 8 90 0 1 1 21 8.2 49 48 1 1 1 I 0 6.7 10 86 0 1 3 21 9.3 28 67 0 3 2 90 11.4 24 71 0 0 5 A 0 5.9 15 82 1 0 2 21 4.7 38 58 1 2 1 1 0 7.5 ] 4 83 1 I 1 21 6.1 27 70 0 2 1 90 8.3 18 78 0 2 2 I 0 7.8 9 90 0 1 0 21 16.2 28 69 0 3 0 .A 0 8.1 7 90 1 0 2 21 4.8 47 50 0 1 2 I 0 5.2 11 86 0 2 1 21 6.1 58 36 1 4 1 I 0 8.7 23 76 0 1 0 21 6.2 45 54 0 1 0 90 21.9 20 75 0 2 3 A 0 7.6 7 93 0 0 0 21 7.5 51 46 1 1 1 I 0 7.0 8 82 0 0 0 21 6.1 30 68 0 2 0 I 0 6.4 12 83 0 1 4 21 16.0 29 68 0 2 1 90 21.3 32 65 0 1 2 A 0 8.7 7 92 0 1 0 21 8.7 30 67 0 2 1 I 0 9.2 17 82 0 1 0 21 6.1 40 57 2 1 0 90 16.6 23 72 0 1 4 I 0 6.8 6 93 0 0 1 21 19.0 47 49 1 2 1 I 0 7.6 9 88 1 2 0 21 6.0 50 44 1 2 3 A 0 9.2 16 82 0 0 2 21 5.2 32 60 2 2 4 I 0 10.1 9 89 1 1 0 21 6.7 53 46 0 1 0 90 Died 56 days * Dose expressed as mg. SLS per kg. body weight per 0.5mi. water per day, 5 days a week. t A = abraded, I -- intact. $ Polys = polymorphonuclear neutrophiles Lymph = lymphocytes Eos -- eosinophiles Mono = monocytes Bas = basophiles.

DERMAL TOXICITY OF SODIUM LAURYL SULFATE 145 TABLE VI--SuBACUTE DE•.MAL STUDIES IN RABBITS TREATED WITH SLS--BLooD CHEMICAL DATA •-•-Blood, rag./100 mi.-- Dose,* Rabbit No. & Skin Period, Urea mg./kg. Sex Treatmentt days Glucose Nitrogen 0 19M A 0 84 8 21 76 15 20M I 0 60 13 21 82 14 90 100 20 21F I 0 71 16 21 89 14 90 90 21 6 1M A 0 74 13 21 96 16 2M I 0 72 15 21 92 14 3M I 0 55 16 21 83 14 90 116 18 4 F A 0 105 16 21 103 14 5 F I 0 104 12 21 105 16 90 85 17 6 F ! 0 94 15 21 94 24 60 7M A 0 95 26 21 89 14 8M I 0 98 21 21 89 17 9M I 0 100 30 21 91 13 90 94 20 10 F A 0 76 13 21 94 20 11F I 0 92 15 21 90 14 12 F I 0 98 13 21 105 15 90 100 18 150 13M A 0 96 14 21 94 15 14M I 0 104 20 2l 78 15 90 88 17 15M I 0 95 18 21 72 9 16M I 0 72 21 21 89 13 17 F A 0 79 16 21 65 15 18 F I 0 87 16 21 84 11 90 Died 56 days * Dose expressed as mg. SLS per kg. body weight per 0.5 mi. water per day, 5 days a week. •' A = abraded, I = intact.