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J. Soc. Cosmet. Chem., 44, 373-374 (November/December 1993) Letter to the Editor TO THE EDITOR: From the original "Comparative studies of skin roughness measurement by image analysis and several in vivo skin testing methods," which appeared in the Journal of the Society of Cosmetic Chemists, 42, pages 385-391 (1991), only the methylene blue test and image analysis were subjected to statistical comment by Wortmann and Wortmann in "The methylene blue and image analysis tests for determining skin roughness: A critical assessment based on data in the literature" (F.-J. Wortmann and G. Wortmann, Journal of the Society of Cosmetic Chemists, September/October 1993). The selected data used by Wortmann and Wortmann originated from studies that took place in 1986-1987. These studies were submitted in 1989 and, as can be seen above, were published in the Journal in 1991 (1). Wortmann and Wortmann have not provided new insights into these methods from experiments of their own. Further work has been carried out on the basis of our findings and experiences with these methods in 1986- 1987. In comprehensive studies involving human volunteers, their accuracy and repro- ducibility have been constantly improved by internal standards and have also been verified by external sources. For instance, the methods that have been established to assess the raw materials and finished products were rated positively in a multi-center study (2). In another study (3), image analysis is described as a method for determining the fine structure, profile, and roughness of the skin. Other studies (4,5) report positive experiences with image analysis in examining the skin surface. When Wortmann and Wortmann come to a negative assessment of the above-mentioned methods in their study based on statistical points of view, this is due to a series of misinterpretations that can easily happen to experts giving theoretical comments with- out experimental experience in this sphere. Their article is based on the refutable assumption that the methylene blue method and image analysis measure identical aspects of skin roughness. The scatter diagram depict- ing the correlation that was established between the methylene blue method and image analysis shows that this assumption is unfounded (1). Our description of the methods explains that the methylene blue method is a staining technique. Staining solutions are able to stain the entire stratum corneum disjunctum, i.e., the information obtained with the methylene blue method originates from the entire volume of the stratum corneum disjunctum. In image analysis, however, the surface topography is recorded. 373