516 JOURNAL OF COSMETIC SCIENCE RSD of six injection times’ peak areas of standard solution 1 and their cross-calibration should not be higher than 2% (Table II). Accuracy, precision, and recovery. The recovery and repeatability varied from 84–101%, from 1.6–5.6%, respectively. Validation of precision also has been done considering intra- and inter-day variation. Data are listed in Table III. Analysis of samples. To evaluate the effectiveness of the established method in a larger range of toothpaste samples, analysis was applied to a total of 17 samples from different manufacturers and batches containing saponins (Table IV). The results showed that there was no significant difference between actual and claimed contents. CONCLUSION A selective and sensitive HPLC-UV method for the simultaneous determination of notoginseng R1, ginsenoside Rg1, Re, Rb1, and Rd in silica matrix toothpastes was developed and validated in this study. This method was successfully applied to 17 samples from different manufacturers and batches. Salt-assisted and ultrasound-assisted methods improved the extraction efficiency. The proposed method has the potential to be applied Table II RSD of Six Injection Times and SD of Cross-Calibration Saponins RSD of 6 injection times/% SD1→2 of cross-calibration/% SD2→1 of cross-calibration/% R1 0.25 0.41 0.41 Rg1 0.08 0.21 0.21 Re 0.10 0.12 0.12 Rb1 0.07 0.77 0.77 Rd 0.07 0.31 0.31 Table III Recovery, Repeatability, Reproducibility, and RSD of Toothpastes Spiked With Saponins (n = 6) Saponins Spiked/ mg kg−1) Founded/(mg kg−1) (RSD/%) Recovery/% D1 D2 D3 R1 320 282 (2.8) 275(2.6) 205(1.8) 86 400 344(1.8) 340(1.9) 332(2.1) 84 480 418 (5.6) 436(4.4) 427(5.5) 89 Rg1 320 310 (2.6) 291 (3.6) 298 (2.4) 94 400 356(1.6) 372(1.8) 360(1.6) 91 480 494 (5.6) 470 (2.7) 484(3.9) 101 Re 320 281 (2.9) 262 (2.6) 270 (2.6) 85 400 364(1.8) 348(1.6) 352(1.6) 89 480 408 (5.7) 417 (5.6) 427 (4.8) 87 Rb1 320 294(2.2) 291(2.1) 275(1.8) 89 400 370(2.1) 376(1.9) 356(2.6) 92 480 456 (5.4) 466(4.5) 460(5.1) 96 Rd 320 294(2.2) 298 (2.6) 301 (2.1) 93 400 366(2.0) 350(1.9) 355(2.3) 89 480 459(5.3) 475(5.0) 451 (4.9) 96

Table IV Saponins Founded in Toothpaste Samples No. Sample-batch number Announced (mg/100 g) Founded (mg/100 g) (RSD/%) R1 Rg1 Re Rb1 Rd R1 Rg1 Re Rb1 Rd 1 Anti-allergic water moistens mint20230209 17.0 55 7.7 55 16.5 18.4(1.4) 56.9(0.9) 7.8(1.3) 56.0(3.9) 16.6(2.1) 2 Active peptide20230209 17.0 55 7.7 55 16.5 15.4(1.5) 54.1(1.4) 8.1(3.3) 53.4(1.8) 13.6(1.4) 3 Active peptide20230210 17.0 55 7.7 55 16.5 15.8(2.6) 55.2(2.6) 8.4(6.3) 54.3(2.5) 13.9(2.5) 4 Good teeth white20230303 10 30 4.2 30 9.0 10.5(2.3) 27(1.9) 3.4(2.8) 32.8(2.0) 9.4(1.3) 5 LAN20230210 7.5 25 3.5 25 7.5 6.5(3.1) 24.5(2.9) 3.4(2.4) 23.6(3.4) 8.4(3.5) 6 LAN20230211 7.5 25 3.5 25 7.5 6.5(4.1) 25.8(5.9) 3.4(5.1) 26.3(4.5) 5.9(4.6) 7 LAN20230212 7.5 25 3.5 25 7.5 7.5(0.2) 26.7(0.2) 3.8(0.6) 25.9(0.3) 7.0(0.3) 8 Fresh mint20230211 7.5 25 3.5 25 7.5 7.7(2.3) 26.6(0.4) 3.6(1.7) 26.4(0.8) 7.1(1.4) 9 Holly20230223 7.5 25 3.5 25 7.5 7.6(4.3) 26.8(4.4) 3.8(4.4) 26.1(4.5) 6.9(4.4) 10 Fresh mint20230213 7.5 25 3.5 25 7.5 7.4(2.9) 26.5(3.3) 3.9(6.0) 25.7(3.9) 6.9(3.4) 11 Fresh mint20230214 7.5 25 3.5 25 7.5 7.3(6.3) 25.9(6.5) 3.7(6.4) 25.3(6.0) 6.8(5.8) 12 Fresh water embellish20230216 7.5 25 3.5 25 7.5 7.7(0.7) 26.9(0.7) 3.9(1.2) 26.4(0.7) 7.3(0.8) 13 Pure and fresh morning dew20230218 7.5 25 3.5 25 7.5 7.2(2.8) 25.7(2.4) 3.8(2.5) 25.2(2.6) 6.7(2.7) 14 Pure and fresh morning dew20230209 7.5 25 3.5 25 7.5 7.5(2.9) 26.4(3.3) 3.9(6.0) 25.7(3.9) 6.9(3.4) 15 Yi chun white20230216 6.0 20 3.0 15 5.0 5.3(0.7) 18.3(1.6) 2.5(1.9) 13.7(0.9) 4.3(1.3) 16 Notoginseng bacteriostatic20221210 1.3 5 0.7 1 2.0 1.1(1.3) 4.9(3.6) 0.5(0.9) 0.8(2.6) 1.5(0.7) 17 Notoginseng toothpaste20221230 1.3 5 0.7 1 2.0 1.4(2.8) 5.4(3.9) 0.7(3.7) 1.1(1.5) 1.7(4.8) 517 SIMPLIFIED SALT-ASSISTED SOLVENT EXTRACTION