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j. Soc. Cosmet. Chem., 28, 79-82 (February 1977) Solubility of cholesterol in isopropyl myristate R. J. HARWOOD and E. M. COHEN, Merck Sharp and Dohme Research Laboratories, West Point, PA 19486 Received March 8, 1976 Synopsis This report describes how the SOLUBILITY of CHOLESTEROL in ISOPROPYL MYRISTATE was de- termined by optical rotation. The procedure described is particularly attractive as compared to other •nalytical procedures used to determine cholesterol content quantitatively with respect to speed and sim- plicity of the measurement. The optical rotation procedure indicates that 5.26 per cent (w/w) cholesterol is '•:: soluble inisopropylmyristate. :. INTRODUCTION Cholesterol and its esters are common constituents of a number of pharmaceutical and cosmetic topical formulations (1-3). In the course of recent laboratory experiments, it was necessary to accurately determine the solubility of cholesterol in isopropyl rnyristate. This report describes how this was determined by optical rotation. In addition, the procedure described herein would appear to be applicable as a general approach to the assay of optically active compounds in lipid and semisolid ingredients of the type generally used for cosmetics, toiletties, and topical pharmaceuticals. 79