Evaluation of sensitisation potential 365 does it make use of intradermal injections as the route of administration it seems particularly well adapted to the testing of finished products whatever their form. ACKNOWLEDGMENT The histological examinations were performed and interpreted by Dr J. Guilaine. RE 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 FERENCES M6thodes officielles d'analyse des cosm6tiques et produits de beaut6. Annexes I, II, III. Arrgt6 du 5 avril 1971. Journal Officiel de la Rdpubliquefrancaise, 21 April 3862 (1971). Brulos, M. F. Mise au point d'une mdthode d'evaluation du pouvoir sensibilisant des produits cosmd- tiques (test proph6tique). Th•se de doctorat de l'Universit6 de Pharmacie. Lyon 14 September 1976. Buehler, E. V. Delayed contact hypersensitivity in the guinea-pig. Arch Dermatol. 91 171 (1965). Draize, J. H. Dermal Toxicity. Dans: Appraisal of the safety of chemicals in foods, drugs and cosmetics, 46-59 (1969). Fischer, A. A. Contact dermatitis. Lea et Febiger, 2nd edition. 1973 Philadelphia. Hardy, J. Allergy, hypersensitivity and cosmetics. J. Soc. Cosmet. Chem. 24 423-468 (1973). Hendeison, C. R. and Riley, E. C. Certain statistical considerations in patch testing. J. Invest. Derm. 6 226, 230 (1945). Knudsen, L. Note on statistical probabilit es of finding hypersensitive subjects in random samples. J. Invest. Dermatol. 6 231-232 (1945). Landsteiner, K. and Jacobs, J. Studies on the sensitisation of animals with simple chemical com- pounds. J. Exptl. Med. 61 643-656 (1935). Magnusson, B. and Hersle, K. Patch test methods III: Influence of adhesive tape on test response. Magnusson, B. and Kligman, A. The identification of contact allergens by animal assay. The maximisation test. J. Invest. Dermatol. 52 268-276 (1969). Magnusson, B. and Kligman, A. Allergic contact dermatitis in the guinea-pig. Identification of contact allergens. Charles C. Thomas, Springfield. 1970. Marzulli, F. N., Carson, T.R. and Maibach, H. I. Delayed contact hypersensitivity studies in man and animals. Proc. Joint. Confer. Cosmt. Sci. Washington D.C.p. 107-122 (1968). Marzulli, F. N. and Maibach, H. I. Antimicrobials experimental contact sensitisation in man. J. Soc. Cosmet. Chem. 24 385-421 (1973). Masters, E. J. Allergies to cosmetic products. N.Y. State. J. Med. 60 1934-1941 (1960). Nilzen, A. Some aspects of epidermal testing in guinea-pig sensitised and not sensitised to 2.4. dinitrochlorobenzene. Acta Dermato Venereol. 32 supplement 29 231-239 (1952). Opdyke, D. J. Monographs on Fragrant Raw Materials. Foods and Cosmet. Toxicology. 11 95- 115, 477-495, 855-876, 1011-1081, (1973) 12 385-405, 517-537, 703-736 (1974) 13 91-112, 449-458,545-554 (1975). Krafr, E. R., Hoch, S. G., Quisno, R. A. and Newcomb, E. A. Evaluating the safety of cosmetics by human patch-test methods. Symposium 'Peau et environnement' J. Soc. Cosmet. Chem. 23 383 (1972). Rochas, A., Guillot, J.P., Martini, M. C. and Cotte, J. Contribution M'6tude de l'influence des parfums sur le pouvoir sensibilisant de bases cosm6tiques. 2•me partie: role du parfum sur le pouvoir sensibilisant de bases cosm6tiques. J. Soc. Cosmet. Chem. This issue. Voss, J. G. Skin sensitisation by mercaptans of low molecular weight. J. Invest. Derm. 31 273 (1958).

J. $oc. Cosmet. Chem. 28 367-375 (1977)¸ 1977 Society of Cosmetic Chemists of Great Britain The influence of perfumes on the sensitising potential of cosmetic bases II. The sensitising potential of perfumes and cosmetic bases A. ROCHAS*, J.P. GUILLOT?, M. C. MARTINIt. and J. COTTEr. Received 4 January 1977 Synopsis A scnsitisation test was used to test a series of cosmetic formulations with and without the addition of perfumes. The reactions obtained were examined and compared with those caused by benzylideneaeetone, a reference sensitiser, which was added to the excipients at the same concentration as the perfumes. INTRODUCTION The development of an animal test technique for evaluating scnsitising potential in the guinea-pig was described in Part I of this paper. The test was devised for use with finished cosmetic preparations for which the Magnusson-Kligman test is not entirely suitable. This section of the paper considers the influence of the addition of several perfumes to typical cosmetic bases. A test substance which has a known allergenic activity was used for comparative purposes. OBJECTIVE Under certain conditions and in certain individuals most substances are potentially allergenic. The chances of any substance being allergenic increases with the complexity of the preparation. With this in mind the research had two aims: to test the eventual sensitising potential of several cosmetic preparations to determine whether the addition of a perfume (of which the sensitising potential was inferior to threshold detection) to a non-sensitising base was likely to lead to a finished product which was a sensitiser and to examine if this phenomenon was related to the cosmetic form used. * Laboratoire de Physiologie B, 8 ave. Rockefeller, 69008 Lyon, France. • Institut Franqais de Recherches et Essais Biologiques, Centre de Lyon, Les Oncins, 69210 L'Arbresle, France. $ Institut de Pharmacie Industrielle, Cosm6tologie, 8 ave. Rockefeller, 69008 Lyon, France. 367