INVESTIGATING PREVENTION OF UV DAMAGE 361 CONCLUSIONS In conclusion, this work has illustrated the utility of Raman spectroscopy for understanding the impact of UV on hair fi ber protein structure, particularly in the decrease of disulfi de band intensity. The protective effect of potential treatment materials was evaluated, and one promising treatment material was identifi ed for further study. Future directions include addressing additional Raman signatures, spatial variation within the hair fi ber, and complementing Raman with infrared measurements. REFERENCES (1) V . Signori, Review of the current understanding of the effect of ultraviolet and visible radiation on hair structure and options for photoprotection, Int. J. Cosmet. Sci., 55, 95–113 (2004). (2) E . Hoting, M. Zimmermann, and S. Hilterhaus-Bong, Photochemical alterations in human hair. I: artifi cial irradiation and investigations of hair proteins, J. Soc. Cosmet. Chem., 46, 85–99 (1995). (3) M . Richena and C. Rezende, Effect of photodamage on the outermost cuticle layer of human hair, J. Photochem. Photobiol. B, 153, 296–304 (2015). (4) M . Richena and C. Rezende, Morphological degradation of human hair cuticle due to simulated sunlight irradiation and washing, J. Photochem. Photobiol. B, 161, 430–440 (2016). (5) A . Kuzuhara, A new method of internal structural analysis of keratin fi bers using Raman spectroscopy, Biopolymer Res. Trends, 61, 49–85 (2007). (6) A. Kuzuhara, Internal structural changes in keratin fi bres resulting from combined hair waving and stress relaxation treatments: a Raman spectroscopic investigation, Int. J. Cosmet. Sci., 38, 201–209 (2015). Figure 5. Dis ulfi de peak height normalized to Phe, CH2 bending, amide I, and CH signatures peak heights.
JOURNAL OF COSMETIC SCIENCE 362 (7) A. Kuzuhara, Analysis of internal structure changes in black human hair keratin fi bers resulting from bleaching treatments using Raman spectroscopy, J. Mol. Struct., 1047, 186–193 (2013). (8) A. Kuzuhara, Internal structure changes in bleached black human hair resulting from chemical treat- ments: a Raman spectroscopic investigation, J. Mol. Struct., 1076, 373–381 (2014). (9) A. Kuzuhara, A Raman spectroscopic investigation of the mechanism of the reduction in hair with thioglycerol and the accompanying disulphide conformational changes, Int. J. Cosmet. Sci., 40, 34–43 (2018). (10) S. Schlücker, C. Liang, K. Strehle, J. DiGiovanna, K. Kraemer, and I. Levin, Conformational differences in protein disulfi de linkages between normal hair and hair from subjects with trichothiodystrophy: a quantitative analysis by Raman microspectroscopy, Biopolymers, 82, 615–622 (2006). (11) A. Kuzuhara, Analysis of structural change in keratin fi bers resulting from chemical treatments using Raman spectroscopy, Biopolymers, 77, 335–344 (2005). (12) A. Kuzuhara, N. Fujiwara, and T. Hori, Analysis of internal structure changes in black human hair keratin fi bers with aging using Raman spectroscopy, Biopolymers, 87, 134–140 (2007). (13) A. Kuzuhara and T. Hori, Analysis of heterogeneous reaction between reducing agents and keratin fi bers using Raman spectroscopy and microspectrophotometry, J. Mol. Struct., 1037, 85–92 (2013). (14) C. M. Pande, FT-Raman spectroscopy: applications in hair research, J. Soc. Cosmet. Chem., 45, 257–268 (1994).
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