Oil part • S-1 Water part MICROBIAL QUALITY CONTROL 83 (70 øC, 20 min) -- (Emulsification)-- (Deaeration) •-- 5-2 Sampling points Result S-1 (--) S-2 (--) s-3 (-) s-4 (-) (-) s-6 (-) S-7 (--) S-3 (70øC, 20 min) S-4 (Cooling) (Storage) (Filling) Figure 8. Testing of final product for microbial contamination Table VII Sampling Time and Sampling Unit in the Course of Actual Production Sampling Time Sampling Unit Untilled product hnmediately after manufacturing Each batch One week after manufacturing Each batch Finished prodnet Immediately after filling Half-day One week after filling Half-day Microbially Free Assurance In order to assure a microbially free product in actual production, samples were taken during the pilot production scale. These were studied as shoxvn in Table VII. Filling is carried out after confirmation that the untilled product passes all tests. Shipment (as a finished product) is done only after it is determined that the product meets all other rigid standards set for it. The tests performed to confirm the number of microorganisms and their species in untilled products and finished products are shown in Fig. 9. RESULTS IN ACTUAL PRODUCTION Check Results of Products Based upon the experimental results obtained, we standardized the micro- bially free techniques in the various stages of manufacturing process and

84 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS I Nutrient agar medium Potato dextrose agar medium Rod Sample I I Cetrimide agar medium I I (+) I Fluid Soybean Casein,- 30øC, 3 days --,Fluid Lactose Digest medium Medium (contain (contain 1% Polysorbate 80) 1% Poly- I sorbate 80) I I I Vogel Johnson Nutrient McConkey agar medium agar medium agar medium I i I I Growth I Gram Stain I No Jrowth ,, ,, OK. Spl•ere I Physiological test I Physiological test Ps. aerug,nosa others St. aureus Figure 9. Microorganisms check-test method for untilled and finished products Table VIII Results of A Emulsion in Actual Production Lot Number Untilled Products Finished Products 1 (--) na= 12, all (--) 2 (--) n = 12, all (--) 3 (--) n = 12, all (--) 4 (--) n = 16, all (--) .5 (--) n = 12, all (--) 6 (--) n = 12, all (--) 7 (--) n---- 16, all (--) 8 (--) n = 12, all (--) 9 (--) n = 4, all (--) 10 (--) n = 4, all (--) •n equals number of samples.