COSMETIC PRESERVATIVES I 267 LITERATURVERZEICHNIS (9) (lO) 01) 02) Aus der Vielzahl der Publikationen sei ohne Anspruch auf Vollstiindigkeit der folgende Abrif• gegeben: a) Berke, P. A., et al., J. Soc. Cosmet. Chem. 29, 757--766 (1978) b) Cowen, R. A., et al., J. Soc. Cosmet. Chem. 27, 467--481 (1976) c) Diehl, K.H., in: ,,Jahrbuch ffir den Praktiker" (1979) 54--71, Verlag f. chem. Industrie H. Ziolkowski KG Augsburg d) Lang, M., et al., Microbios 7 (28), 199--207 (1973) e) O'Neill, J. J., et al., J. Soc. Cosmet. Chem. 30, 25--38 (1979) f) Nowak, G. A., in: Kosmetik Jahrbuch 1979, Verlag fiir chem. Industrie, H. Ziolkowski KG Augsburg g) Parker, M.S., et al., Riv. Ital. Essenze, Profumi, Piante off., Aromi, Saponi Cosmet., Aerosol 1972, 54 (5), 363--366 h) Poelman, M. C., et al., NN pharm. fr., 33, 11,551--557 (1975) i) Richardson, E.L., Costa. and Toiletries 92, 85--86 (1977) j) Wallhiiut•er, K. H., Farbe u. Lack 82, 100 (1976) k) Wallhi/uf•er, K. H., Parf. u. Kosm. 60, 116--117 (1979) (2) Siebert, G., Parf. u. Kosm. 60, 239--244 (1979) (3) Lingnau, J., Parr. u. Kosm. 60, 118--122 (1979) (4) a) Nowak, G. A., Parr. u. Kosm. 60, 8--18 (1979) b) Kowatsch, R., Parr. u. Kosm. 59, 8, 255--263 (1978) (5) a) Kudernatsch, H., Parf. u. Kosm. 60, 137--138 (1979) b) Bellinger, H., Parf. u. Kosm. 58, 57--60 (1977) (6) Bellinger, H., Parf. u. Kosm. 60, 139--149 (1979) (7) Kosmetik Verordnung v. 16.12. 1977 (BGB1. I S. 2589) (8) Butler, N.J., in: ,,Biodeterioration of Materials", S. 269 if., Elseviers Publishing Co. Ltd. Am- sterdam, London, New York Wallhi/uger, K. H .... Sterilisation -- Desinfektion -- Konservierung" S. 385 2. Auflage Georg Thieme Verlag Stuttgart (1978) Gesetz fiber den Verkehr reit Lebensmitteln, Tabakerzeugnissen, Kosmetischen Mitteln und sonstigen Bedarfsgegensti/nden vom 15.8. 1974 5 24 a) DuPont, H., Medicine 52, 265--270 (1974) b) Stamer, R.Y., et al., in: ,,The microbial world", S. 786 if. Prentice-Hall, Inc., Englewood Cliffs, New Jersey (1976) Stache, H., Tensid-Taschenbuch, Hanser-Verlag Mfinchen (1979)

j. $oc. Cosmet. Chem., 31,269-271 (September/October 1980) Effect of Sapindus extract on hair development C. L. ANAND, Phylum Therpicare Ltd., 224 [Vest Regent Street, Glasgow G2, Great Britain, J. R. HILLMAN, Department of Botany, University of Glasgow, Glasgow G12, Great Britain, E. M. PANTELOURIS, and CLARE P. BARTLET, Department of Biology, University of Strathclyde, Glasgow GI, Great Britain, Received August 30, 1979. Synopsis Extracts of the fruit of Sapindus trifoliatus contain some factor(s) which, if locally applied, appeared to prolong the 'anagen' stage of hair growth in the skin of mice. INTRODUCTION Extracts of the fruit of the tree 'reeta' (Sapindus trifoliatus) are widely used in India for cleaning woolien fabrics, with excellent results. It was thought interesting therefore to test its usefulness as a cleanser of hair. In such tests--which are in hand and will be reported in due course--it appeared that the extract was beneficial in cases of dandruff. Prior to these tests in man and as a test for toxicity or side-effects, the extract was applied to the skin of mice, and the treated skin was examined histologically. EXPERIMENTAL Extracts were prepared by soaking, air-dried pericarp of S. trifoliatus fruit for 24 hr in 70% propan-2-ol the solution was then filtered and stored in the dark. In all experiments the treatment consisted of daily applications of extract and control solutions to shaved patches of skin chosen symmetrically on either side of the dorsal mid-line of each mouse. Treatment was continued for specific periods (4 to 5 weeks) on animals of known ages. The animals were then killed and samples of the skin from the treated areas were taken for detailed histology. In some instances the skin samples were sectioned in two directions, parallel and at right angles to the midline. At least 10 sections were picked out at 240 /xm intervals from the ribbon of serial sections available for each skin sample. 269