JOURNAL OF COSMETIC SCIENCE 60 The test materials applied to the patches were cream, lotion, and 2% bath cream. The cream and lotion were similar in their main ingredients, but differed in dosage. The 2% bath cream was prepared using 2 g of the bath cream diluted with 98 g of distilled water. Main ingredients of the three test materials were shown in Table I. Each patch had one positive control and one negative control. One percent sodium lauryl sulfate (SLS, purity 99%, made by Sigma, St. Louis, MO) solution served as the positive control. Blank patches with a pad served as the negative control. Figure 3. Band-Aid semiocclusive. Figure 4. Webril occlusive.

IRRITANCY PATCH TEST COMPARISON 61 METHOD Patches dosed with test materials were applied laterally between the shoulder blades on the upper back, but not directly over the spine. Each type of patch was placed in a sepa- rate row. Test samples were added in the same sequence according to Figure 6. Test and control materials were applied to each patch in 20 μl. Patches were applied for approximately a 24-h period, then removed and discarded by the dermatologist approxi- mately 2 h before grading. After an evaluation, materials were reapplied to their assigned sites. These procedures were performed daily for 11 d, with fi nal evaluations performed after the fi nal patch removal on day 12. All induction patches were applied to the same sites, unless the cumulative grade of reac- tion was more than three or the adhesive necessitated removal. Figure 5. Webril semiocclusive. Table I Main Ingredients of the Three Test Materials Cream Lotion Bath cream Water Water Water Glycerine Glycerine Sodium benzoate Cetyl alcohol Cetyl alcohol Cocamidopropyl betaine Cocoglycerides Glycine soja oil Sodium laureth sulfate Glycine soja oil Helianthus annuus seed oil Sodium lauroamphoacetate Helianthus annuus seed oil Zea mays starch Polysorbate 20 Dimethicone Dimethicone Polyethylene glycol-150 distearate Zea mays starch Phenoxyethanol Citric acid Potassium cetyl phosphate Ethylhexylglycerin Hydrogenated palm glycerides Potassium cetyl phosphate