306 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS sidered with respect to each sample type. In each case, the final colorless aqueous solution is the "Prepared Sample" in which the allantoin content is determined by either of the methods described above. The sample size is selected on the basis of the concentration of allantoin (or aluminum allantoinate) in the preparation. For example, in the case of lipsticks, generally 0.075 to 0.1% allantoin is used, and the sample size has to be such as to insure a sufficient amount of allantoin in the final extract. Also, in the case of lipsticks, suppositories and nonaqueous preparations, it is more difficult to extract allantoin from the sample, and the larger sample minimizes the chance of error in the final results. On the other hand, extraction of allantoin, etc., from lotions and hydro- alcoholic formulations is easier, and smaller samples may be employed. In other words, it is best to select that weight of sample which would yield enough allantoin (or aluminum allantoinate) in the final extract to attain reproducible results. 1. Lipstick with ztllantoin. Suspend 25.0 g. of the lipstick in 50 ml. of distilled water. Heat the solution to boiling and dissolve 5 g. of am- monium sulfate in the hot solution. Add 5 g. of activated carbon and mix thoroughly. Immediately filter the hot solution through a pre- moistened double fluted filter paper. If the flitrate is not colorless, refilter with additional activated carbon. The clear colorless tiltrate is then transferred to a separatory funnel and treated by either of the procedures outlined above. 2. Suppository with ztluminum Dihydroxyallantoinate. Suspend 25.0 g. of the suppository formula in 50 ml. of hot distilled water and add 5 ml. of concentrated hydrochloric acid. Place the solution in a boiling water bath. Dissolve 5 g. of ammonium sulfate in the hot solution and allow it to stand in the water bath 5-10 minutes. Add 2 g. of activated carbon and mix thoroughly. Filter through a premoistened double fluted filter paper. The clear colorless flitrate is transferred to the separatory funnel and treated by either of the procedures outlined above. 3. ,4erosol Shave Cream with ,4llantoin. Mix 15.0 g. of the shave cream with 40 ml. of distilled water and dissolve 5 g. of ammonium sulfate in the solution. With slow constant stirring, gradually heat the solution to boiling. Add sufficient boiling water to bring total volume to 50 ml. Boil 1-2 minutes and add 1 g. activated carbon. Filter while hot through a premoistened double fluted filter paper. Transfer the flitrate to the separatory funnel and determine the allantoin content of the sample by either of the above procedures. 4. zterosol Shave Cream with ztluminum Dihydroxyallantoinate. Mix 15.0 g. of the cream with 40 ml. of distilled water. Add 5 ml. of concen- trated hydrochloric acid and 5 g. of ammonium sulfate. Heat in a boiling water bath for 5 minutes with constant stirring. Bring to a volume of 50

DETERMINATION OF ALLANTOIN AND THE AL ALLANTOINATES 307 ml. with hot distilled water. Add 1 g. of activated carbon and filter while hot. The clear colorless flitrate is transferred to the separatory funnel, and the allantoin content is determined by either of the above methods. 5. Cream or Ointment Containing/tllantoin or/tluminum Chlorhydroxy- allantoinate /tlone or in Combination with/tntiseptics, /tntibiotics, Sulfur, Resorcin, etc. Thoroughly mix 25.0 g. of the cream with 50 ml. of dis- tilled water. Dissolve 5 g. of ammonium sulfate in the mixture. Bring to a volume of 75 ml. and boil for 2-5 minutes. Add 3 g. of activated carbon, mix thoroughly and filter immediately. Transfer the flitrate to the separatory funnel and determine the allantoin content of the sample by either of the above methods. 6. Cream or Ointment with/tluminum Dihydroxyallantoinate /tlone or in Combination with/tntiseptics,/tntibiotics, etc. Thoroughly mix 25.0 g. of the cream with 50 ml. of hot distilled water. Add $ g. of ammonium sulfate and 5 ml. of concentrated hydrochloric acid. Add sufficient hot distilled water to bring the volume of the mixture to 75 ml. and place in a boiling water bath for 5-7 minutes. Add 2 g. of activated carbon and filter while hot. Transfer the flitrate to the separatory funnel and deter- mine the allantoin content of the sample by either of the above methods. 7. Shampoo with /tllantoin /tlone or in Combination with /tntiseptics, Sulfur, etc. A 25.0 g. sample of the shampoo is treated by the same pro- cedure as the cream or ointment in (5) above. 8. Lotions with /tllantoin or /tluminum Chlorhydroxyallantoinate. A 25.0 g. sample of the lotion is treated by the same procedure as the cream or ointment in (5) above. 9. Lotion with/tluminum Dihydroxyallantoinate. A 25.0 g. sample of the lotion is treated by the same procedure as the cream with aluminum dihydroxyallantoinate in (6) above. 10. /tfter-shave Lotion with /tllantoin or /tluminum Chlorhydroxy- allantoinate. A 25.0 g. sample of the lotion is treated by the same pro- cedure as the cream or ointment in (5) above. 11. Talcums and Compressed Powders with /tllantoin or /tluminum Chlorhydroxyallantoinate. Thoroughly mix 25.0 g. of the talc or powder with 50 ml. of hot distilled water. Dissolve $ g. of ammonium sulfate in the mixture. Add sufficient hot distilled water to give a total volume of 75 ml. Boil for several minutes. Add 2 g. of activated carbon and mix thoroughly. Boil for 1 minute and filter immediately. Transfer the flitrate to the separatory funnel and determine the allantoin content by either of the methods described above. 12. Talcums and Compressed Powders with ztluminum Dihydroxyal- lantoinate ztlone or in Combination wtth /tntiseptics, Sulfur, etc. Thoroughly mix 25.0 g. of the talcum or powder with 50 ml. of hot distilled water and