IRRITANCY TO THE RABBIT EYE MUCOSA OF CREAM SHAMPOOS 665 3. Mildly irritant - conjunctival reaction, accompanied by slight corneal and/or iridial reaction (maximum score of 5), the latter persisting for up to 5 days. 4. Moderately irritant- conjunctival reaction accompanied by moderate corneal and/or iridial reaction (maximum score of 10), the latter persisting for up to 5 days. 5. Severely irritant - conjunctival reaction accompanied by more persistent and severe injury to the cornea and/or iris (score above 10), effects persisting for more than five days. RESULTS To facilitate comparison between results obtained by the different test procedures, the data have been expressed as mean values per rabbit and are listed for each shampoo in Tables I-V. Significant deviations from the mean value in the case of individual animals are referred to in the text that follows. Table I Mean scores/rabbit of reactions elicited by shampoo A Days after treatment Da.ys of Test No. of maxtmum rabbits 1 2 3 I 4 7 persistence (a) FDA 6 11 13 13 13 8 134-q- (b) Draize Unwashed 3 11 15 17 15 7 15 Washed at 2 sec 3 8 $ 3 3 0 4 V•rashed at 4 sec 3 9 5 4 3 0 4 (c) Dilution 6 9 6 4 2 1 7 (d) HRC screen Unwashed 2 11 11 11 13 14 134-t- Washed at 4 sec 2 9 6 4 3 0 4 Table II Mean scores/rabbit of reactions elicited by shampoo B Test (a) FDA (b) Draize Unwashed Washed at 2 sec Washed at 4 sec (c) Dilution (d) HRC screen Unwashed Washed at 4 sec No. of rabbits 16 Days after treatment 2 3 4 10 Da.ys of maximum persistence

666 JOURNAI. OF THE SOCIETY OF COSMETIC CHEMISTS Table III Mean scores/rabbit of reactions elicited by shampoo C Days after treatment Da.ys of Test No. of maximum rabbits 1 2 3 4 7 persistence ,, (a) FDA 6 12 10 8 7 7 40 (b) Draize Unwashed 3 13 9 6 7 9 40 Washed at 2 sec 3 9 4 $ 2 0 4 Washed at 4 sec 3 6 4 $ 1 0 4 (c) Dilution 6 8 4 2 1 1 7 (d) HRC screen Unwashed 2 13 12 8 8 7 40 •Vashed at 4 sec 2 6 4 4 2 0 4 Table IV Mean scores/rabbit of reactions elicited by shampoo D Days after treatment Da.ys of Test No. of maximum rabbits 1 2 3 4 7 persistence (a) FDA 6 11 9 12 7 6 70 (b) Draize Unwashed 3 11 7 11 5 3 17 Washed at 2 sec 3 4 1 0 0 0 2 XVashed at 4 sec 3 6 2 0 0 0 2 ic) Dilution 6 5 2 1 0 0 3 '•d) HRC screen Unwashed 2 11 13 14 11 11 70 Washed at 4 sec 2 5 1 0 0 0 2 Table V •V[ean scores/rabbit of reactions elicited by shampoo E I Days after treatment Days of Test I No. of I maximum . rabbits 1 2 3 4 7 persistence (a) FDA 6 • 12 10 8 6 6 45 (b) Draize Unwashed I 3 11 9 7 4 3 9 Washed at 2 sec I 3 2 2 1 0 0 3 Washed at 4 sec • 3 3 2 1 0 0 3 (c) Dilution 6 [ 8 8 4 3 1 7 (d) HRC screen Unwashed 2 12 11 9 8 11 45 Washed at 4 sec 2 4 3 1 0 0 3