104 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS L Figure 3. The same follicular biopsy as in Figure 2 viewed under polarized illumination. Note the contrast between the horny impactions and the background. more intense, standing out from the background (Figure 3). This contrast between the impactions and the background allows the samples to be evaluated by image analysis for total number and size distribution of impactions. A task (program) for evaluating FB slides under polarized illumination was developed that automatically counts the number, area, and size distribution of horny impactions. An IA task consists of the following basic steps: 1. Image capture (digitizing) 2. Segmentation 3. Object detection 4. Measurement/data storage 5. Analysis The task automatically controls what objects to evaluate (based on intensity range), what type of editing to perform, and what measurements to make and record. An example of a task used to evaluate follicular biopsy slides is illustrated in Figure 4. Image analysis data was obtained from the FB slides of the 12 panelists for each test sample. The follicular biopsies were evaluated at 15 x, giving a field of view 9.4 mm x 10.0 mm. The measurements are in units of pixels for comparative purposes. RESULTS AND DISCUSSION When looking at the individual data from each panelist for sample A (cleansing masque), we see a relationship between the interpretive scores and the number of objects

IMAGE ANALYSIS 105 { iO•ig•nal task file COMEDO7.TSK } { Task list for GENIAS25 2.1 } MAIN MENU Capture CAPTURE Correct Change COMEDO CAPTURE Quit MAIN MENU Grey GREY OPS Linear LINEAR FILTERS Mean LINEAR FILTERS Quit GREY OPS Quit MAIN MENU Threshold THRESHOLD Manual 50 {SLICE : LOW } 63 {SLICE : HIGH } THRESHOLD Quit MAIN MENU Binary BINARY OPS Object-based OBJECT-BASED OPS Delete DELETE OBJECTS Area 0.00000E+00 { Minimum? 1.00000E+01 { Maximum? Inc lus ive DELETE OBJECTS Quit OBJECT-BASED OPS Quit BINARY OPS Quit MAIN MENU Measure Objects FIELD MEASUREMENTS Quit 0 { Nesting levels? [0 = no nesting] } Yes MEASUREMENTS 1 Select-all MEASUREMENTS 1 Quit MAIN MENU Quit Yes END Figure 4. Anim•eanalysisprogram (t•klist) used toevaluate •11icularbiopsyslides.