JOURNAL OF COSMETIC SCIENCE 388 lotion was found to be 194 ± 14 μg/ml (range: 180–212 μg/ml). Recovery of spiked hi- nokitiol from the lotion was 91.8 ± 3.7% (range: 84.5–98.0%). CONCLUSION We have developed a simple HPLC–UV method for the determination of hinokitiol after precolumn derivatization with NBD-F. Although the sensitivity of our method can be classed as only moderate compared with other reported methods, it is superior to that of our previous method. The newly developed HPLC method is simple, convenient, and suffi ciently sensitive for routine assay of hinokitiol, for example in quality control for personal care products. REFERENCES (1) J. Nault, A capillary gas chromatographic method for thujaplicins in western red cedar extractives, Wood Sci. Technol., 21, 311–316 (1987). (2) Y. Inamori, S. Shinohara, H. Tsujibo, T. Okabe, Y. Morita, Y. Sakagami, Y. Kumeda, and N. Ishida, Antimicrobial activity and metalloprotease inhibition of hinokitiol-related compounds, the constitu- ents of Thujopsis dolabrata S. and Z. hondai MAK, Biol. Pharm. Bull., 22, 990–993 (1999). (3) Y. Inamori, Y. Sakagami, Y. Morita, M. Shibata, M. Sugiura, Y. Kumeda, T. Okabe, H. Tsujibo, and N. Ishida, Antifungal activity of hinokitiol-related compounds on wood-rotting fungi and their insec- ticidal activities, Biol. Pharm. Bull., 23, 995–997 (2000). (4) E. L. Johnson and A. J. Cserjesi, Gas-liquid chromatography of some tropolone-TMS ethers, J. Chro- matogr., 107, 388 (1975). (5) F. Hanafusa, K. Nakamura, S. Togano, and T. Ohta, Determination of hinokitiol in cosmetic products by HPLC, Bunseki Kagaku, 38, 124–128 (1989). (6) M. Endo, T. Mizutani, M. Matsumura, M. Moriyasu, M. Ichimaru, A. Kato, and Y. Hashimoto, High- performance liquid chromatographic determination of hinokitiol in cosmetics by the formation of difl u- oroborane compounds, J. Chromatogr., 455, 430–433 (1988). (7) L. Dyrskov, B. W. Strobel, B. Svensmark, and H. C. Hansen, Beta-thujaplicin: New quantitative CZE method and adsorption to goethite, J. Agric. Food Chem., 52, 1452–1457 (2004). (8) Y. Higashi, M. Sakata, and Y. Fujii, High-performance liquid chromatography with dual-wavelength ultraviolet detection for measurement of hinokitiol in personal care products, J. Cosmet. Sci., 60, 519– 525 (2009). (9) K. Imai, Analytical chemical studies on high-performance recognition and detection of bio-molecules in life, Yakugaku Zasshi, 123, 901–917 (2003). Table III Level of Hinokitiol in a Skin Lotion and Recovery of Spiked Hinokitiol Assay Amount (μg/ml) Recovery (%) Added (50.0 μg) Added (100 μg) Day 1 204 88.8 93.2 Day 2 180 94.1 98 Day 3 212 91.3 91.9 Day 4 192 92.2 94.4 Day 5 183 84.5 89.2 Average ± S.D. (R.S.D.) 194 ± 14 (7.2%, n = 5) 91.8 ± 3.7 (4.0%, n = 10)
HPLC DETERMINATION OF HINOKITIOL 389 (10) Y. Higashi, S. Nakamura, H. Matsumura, and Y. Fujii, Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fl uoro-7-nitro-2,1, 3-benzoxadiazole as a fl uorescent derivatization reagent, Biomed. Chromatogr., 20, 423–428 (2006). (11) Y. Higashi, M. Sakata, and Y. Fujii, Simultaneous determination of the N-dealkylated metabolites of four butyrophenone-type agents in rat plasma by HPLC with fl uorescence detection after pre-column derivatization with 4-fl uoro-7-nitro-2,1,3-benzoxadiazole, J. Liq. Chromatogr. Relat. Technol., 30, 2747– 2754 (2007). (12) T. Fukushima, J. Kawai, K. Imai, and T. Toyo’oka, Simultaneous determination of D- and L-serine in rat brain microdialysis sample using a column-switching HPLC with fl uorimetric detection, Biomed. Chromatogr., 18, 813–819 (2004). (13) Y. Higashi, R. Gao, and Y. Fujii, Determination of fl uoxetine and norfl uoxetine in human serum and urine by HPLC using a cholester column with fl uorescence detection, J. Liq. Chromatogr. Relat. Technol., 32, 1141–1151 (2009). (14) T. Toyo’oka, T. Mantani, and M. Kato, Characterization of labelling and de-labelling reagents for detec- tion and recovery of tyrosine residue in peptide, Biomed. Chromatogr., 17, 133–142 (2003). (15) Y. Higashi and Y. Fujii, HPLC–UV analysis of phenol and chlorophenols in water after pre-column derivatization with 4-fl uoro-7-nitro-2,1,3-benzoxadiazole, J. Liq. Chromatogr. Relat. Technol., 32, 2372– 2383 (2009). (16) Y. Higashi and Y. Fujii, HPLC–UV analysis of eugenol in clove and cinnamon oils after pre-column derivatization with 4-fl uoro-7-nitro-2,1,3-benzoxadiazole, J. Liq. Chromatogr. Relat. Technol., 34, 18–25 (2011). (17) Y. Higashi and Y. Fujii, Determination of kojic acid in a skin-whitening cosmetic by HPLC–UV analy- sis after pre-column derivatization with 4-fl uoro-7-nitro-2,1,3-benzoxadiazole, J. Cosmet. Sci., 63, 205–212 (2012).
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