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J. Cosmet. Sci., 72, 163–171 (March/April 2021) 163 Effectiveness of the Disinfection of Reusable Make-Up Applicators—Initial Experiences P IOTR ZALĘCKI, JOANNA TWARDOWSKA, DANUTA NOWICKA, and WALDEMAR ANDRZEJEWSKI, Fa culty of Physiotherapy, University School of Physical Education, Wroclaw 51-612, Poland (P. Z., J.T., W.A., D.N.), Department of Dermatology, Venereology and Allergology, Wroclaw Medical University, Wroclaw 50-368, Poland (D.N.) Accep ted for publication December 15, 2020. Synop s is Make- up accessories may be subjected to bacterial contamination due to their contact with many surfaces, including the client’s skin. Disinfection prevents the spread of infection, but the capacity to eliminate microorganisms varies among disinfection methods. Little is known about disinfection in beauty salons therefore, we aimed to compare the effectiveness of disinfectants for reusable make-up applicators. We tested reusable make-up applicators (natural brush, synthetic brush, and sponge) with disinfectants (bactericidal soap, 70% ethanol, and Hydro Sept solution). The number of microorganisms present before and after disinfection using the plate method was assessed. In comparison to water, bactericidal soap reduced the number of bacterial colonies. There were 418 versus 83 colonies in natural brush samples, 1,162 versus nine colonies in synthetic brush samples, and 617 versus three colonies in sponge samples. Ethanol eliminated 100% of bacterial growth. Disinfection was more effective on applicators used for dry products (natural brush) than for wet products (sponge, synthetic brush). We conclude that disinfection is a complex process and depends on the type of make-up applicator being disinfected and the type of cosmetics being used. Further research on the selection of appropriate disinfection procedures for individual applicants and for those who prepare makeup is needed. INTRO D UCTION Reusa ble make-up applicators come into contact with many surfaces, including in par- ticular the client’s skin, which contributes to their contamination with various microbes (1). In the case of cosmetics, microorganisms such as Candida albicans, Pseudomonas aeru- ginosa, Serratia marcescens, Escherichia coli, Burkholderia cepacia, Klebsiella oxytoca, Staphylococ- cus aureus, and Enterobacter gergoviae pose the greatest risk and negatively affect the health of consumers (2). Data from the literature indicate that products and make-up accessories may be subjected to bacterial contamination, depending on the number of people using the cosmetic, the composition of the cosmetic, and the users’ hygiene habits. Wilson et al. Address all correspondence to Danuta Nowicka at danuta.nowicka@umed.wroc.pl