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J. Cosmet. Sci., 62, 305–316 (May/June 2011) 305 Evaluation of anti-cellulite effi cacy: A topical cosmetic treatment for cellulite blemishes—A multifunctional formulation ADELE SPARAVIGNA, GIANCARLO GUGLIELMINI, STEFANO TOGNI, ALDO CRISTONI, and GIADA MARAMALDI, Derming, Monza (MB) (A.S.), Sinerga, Pero (MI) (G.G.), and Indena, Milan, (S.T., A.C., G.M.), Italy. Synopsis Cellulite is thought to affect 80-90% of postpubertal women, and is considered much of a cosmetic concern by the large majority of them. In this study, the objective was to assess the effi cacy of a topical cosmetic product containing various active ingredients of botanical origin on cellulite blemishes on female volunteers affected by fat accumulations, as well as by slight-to-moderate cellulite in the lower limbs. The assessment was performed by means of various objective evaluations, including contact thermography, morphometric measures of thigh circumference, and microcirculation evaluation. The obtained results indicate that the use of synergistic botanical standardized extracts, through the exploitation of different mechanisms of action and acting on different biological targets, provides visible and measurable results in the improvement of cellulite signs and symptoms. INTRODUCTION Cellulite onset and recognition as a real pathology is still controversial, as well as its exact causes. On the one hand, it has been recently described as a physiological condition aimed to maximize adipose retention in order to ensure adequate caloric availability during pregnancy and lactation (1), but cellulite is also a complex problem, involving several different factors and mechanisms, such as metabolic imbalances, alterations in connective tissue structure, genetic factors, infl ammatory conditions, reduced microcirculation, and hormonal factors. Although genetic predisposition to cellulite has been related to a par- ticular polymorphism in the angiotensin-converting enzyme and hypoxia-inducible fac- tor 1A genes (2), diet and lifestyle also play an important role in the improvement or worsening of the aspect of cellulitic areas. From a metabolic standpoint, during the onset of cellulite, adipocytes become engorged with lipids and cluster together while an excess of subcutaneous fl uids is retained. Also, Address all correspondence to Giada Maramaldi at Giada.maramaldi@indena.com Stefano Togni, Aldo Cristoni, and Giada Maramaldi work at Indena SpA, the company that produces the active ingredients used in the trail. Giancarlo Guglielmini works at Sinerga SpA, the company that manufac- tured the batches of active treatment and placebo used in the trial.