274 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS TABLE 1--REcoRD or WEIGHTS Or RABBITS USED IN TOXICITY TESTS ß -Days Total Rabbit 1 5 10 15 20 21 Gain 1 2.920 3.100 3,130 3.105 2 2.720 2.710 2.850 2.970 3 2.210 2.460 2,560 2.655 4 2,525 2.620 2.645 2.875 5 3.250 3,450 3.480 3.715 6 2.710 2.855 2.870 2.975 7 3.470 3.570 3.600 3.695 8 2.750 2.795 2.850 2.995 9 3.020 3.075 3.145 3.185 10 2.880 2.930 2.915 2.950 ll 2.890 3.070 3.270 3.495 12 3.085 3.150 3.245 3.420 13 1.880 1.935 2.105 2.200 14 2.335 2.450 2.465 2 620 15 2.170 2.435 2.535 2.735 16 2.765 2 820 2.610 2.635 17 3.365 3.405 3.545 3.790 18 2.160 2.390 2 385 2.440 19 2 390 2.675 2.720 2.965 20 1.885 1.965 2.120 2.395 3.115 3.695 2. 870 2. 895 3. 600 3.190 3.700 2 990 3 320 2 935 3 620 3 435 2 260 2 755 2 800 2 605 3 830 2.405 2.900 2.410 3 115 0.195 3.050 0.330 2.875 O.66O 2.935 0.410 3.750 0.SO0 3.065 0.355 3.700 0.225 2.965 0.215 3 315 0.295 2.955 0.075 3.615 0.925 3.530 0.445 2.300 0.420 2.780 0.445 2.805 0.635 2.610 0.155 3.780 0,415 2.455 0.295 2.960 0.570 2.410 0.525 Rabbits IV, V, VIII, and XI1 were inuncted with beta-mercaptopro- pionic acid IX, XVII, II, and VI were inuncted with mercaptosuccinic acid XI, XVI, VII, and X were inuncted with taurine III, I, XV, and XIII were inuncted with thioglycolic acid XIV, XVIII, XIX, and XX were controls. These rabbits were examined before weighing to deter- mine the degree of irritation. An arbitrary standard was set up as a meas- ure of irritation, and the degree was indicated by numbers as follows: zero indicated no effect on the skin, 1 a slight reddening and in t- a deep red 2 a slight scab formation and 2+ a severe scab formation covering most of the area. At the end of 20 days the results were as follows: Thioglycolic acid had a total of fifty-six 2's and two 2q-'s. This was the most toxic of the compounds used. Mercaptosuccinic acid was next with a total of twenty- five 2's, with taurine having a total of nineteen 2's. The beta-mercapto- propionic acid had a total of eight 2's and the control had none. (See Table 2.) The hemoglobin content in the rabbits was determined by the Oster- berg's Modification method, a combination of Kennedy's and Wong's methods (9). The blood samples were taken every five days and one taken before the test began. The hemoglobin content of the rabbits did not differ more than 8.3,0/o and the deviation may have been due to the instrument or the operator. The five sets of creams were applied by patch tests on the inner part of the forearm of humans as follows: One ml. of the cream, measured out with a hypodermic syringe, was applied to band-aids in such a manner that ap-

ORGANIC SULFUR COMPOUNDS IN COLD WAVES -F 275