STING POTENTIAL OF SHAMPOOS 587 Table l Dose Response Curves Obtained With 8 Experimental and 3 Commercially Available Shampoos Per Cent Number of Mice Per Cent Shampoo Aqueous Concentration Writhing/Dosed • Writhing WD.• (95 Per Cent C.L.) •' "B" 08B 14F 14G 08A 14E 25S 25M 101 2.0 3/10 30 3.0 5/10 5O 4.0 8/15 53 5.0 13/15 87 1.0 0/10 0 2.O 2/10 2O 4.0 10/10 100 1.0 0/10 0 2.O 3/10 30 4.0 10/10 100 1.0 1/10 10 2.O 4/10 4O 4.O 9/10 9O 0.5 0/10 0 1.0 1/10 10 2.0 5/10 50 4.0 8/10 8O 1.0 0/10 0 2.0 8/10 80 4.0 9/10 90 1.0 0/10 0 2.O 8/10 8O 4.O 10/10 100 0.25 0/10 0 0.5 8/10 80 1.0 9/10 90 2.0 10/10 100 0.125 0/10 0 O.25 6/10 6O 0.5 10/10 100 1.0 5/5 100 2.O 5/5 100 4.O 5/5 100 0.125 1/10 10 0.25 6/10 60 O.5 9/10 9O 1.0 10/10 100 2.O 5/5 100 4.0 5/5 100 0.125 1/10 10 0.25 8/10 8O 0.5 10/10 100 1.0 5/5 100 2.0 5/5 lOO 4.O 5/5 100 3.0 (1.7-5.2) 2.5 (2.o-a.2) 2.2 (1.8-2.7) 2.2 (1.4-3.4) 2.1 (1.5-3.1) 1.7 (1.1-2.5) 1.6 (1.3-2.0) 0.42 (0.26-0.67) 0.24 (0.20-0.28) 0.23 (0.15-0.36) 0.19 (0.14-0.25) '•Dose: intraperitoneal injection with 0.2 ml of each shampoo concentration. "WD•0: Per cent aqueous concentration required to produce writhing syndrome (discom- fort) in 50 per cent of the test mice.

588 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS o o