EVALUATING ANTIDANDRUFF AGENTS Table I Twice Weekly Three-Week Treatment Schedule 575 Day Pretreatment --7 --4 0 3 Treatment Posttreatment 7 10 14 17 21 24 28 Bland shampoo Test shampoo Grade Corneocyte count x x x x x x x x x x x x x x x x x x x x Corneocyte$ I 2 Grades 4 Figure I. Graphic representation of data in Table I, expressed as percentage reductions from original •level. Effectiveness of both shampoos is immediately apparent in contrast to control. Grades and counts are still significantly decreased 1 week after the last sham- poo: (x) control (o) 2 per cent ZPT (') 2.5 per cent SeS The results obtained by the use of three agents are shown in Fig. 1 and Table II. The bland shampoo produced no change in the clinical grade or corneocyte count. A significant reduction in the count and grade was evident in i week with both 2.5 per cent selenium sulfide (SeS) and 2 per cent zinc

576 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Table II Twice Weekly-Three-Week Schedule Dandruff Shampoo Day 0 7 14 21 28 A a Control shampoo •P10 Grade 5.2 4.9 5.0 4.9 4.9 Corneocytes GM d 1,013,000 860,000 894,800 88'3,900 898,600 SD e 0.14 0.13 0.08 0.12 0.16 B b +2 per cent ZPT •p12 Grade 5.2 4.6 3.4 2.3 3.3 Corneocytes GM 1,074,000 785,900 658,900 674,100 695,000 SD 0.10 0.09 0.12 0.12 0.11 ++2.5 per cent SeS Grade Corneocytes A Control shampoo gp 12 Corneocytes 2 per cent ZPT •p13 Comeocytes 2.5 per cent SeS •p12 Corneocytes 5.4 4.2 3.1 2.5 2.6 GM 1,205,000 683,100 444,600 340,200 444,000 SD 0.22 0.21 0.13 0.24 Non-Dandruff GM 788,300 707,000 733,000 767,000 794,000 SD 0.25 0.23 0.21 0.25 0.22 GM 833,900 463,500 549,600 489,300 502,900 SD 0.18 0.16 0.18 0.31 0.19 GM 799,300 589,300 489,100 319,400 384,300 SD 0.11 0.24 0.16 0.24 0.15 "Johuson & Johnson Baby Shampoo. t'Hcad & Shoulders. ' Selsun. "Geometric mean. '•Standard deviation: the values enclosed are statistically different from the pretreatment figures. Table III Intensive Shampooing Schedule Day Pretreatment Treatment Posttreatment --7 --4 0 1 2 3 4 5 6 7 8 9 10 11 12 16 20 24 Bland shampoo x x Test shampoo x x x x x x x x x x x Grade x x x x Corneocyte count x x x x