38 JOURNAL OF COSMETIC SCIENCE of cleansers by identifying products that might induce stratum corneum damage. Also, pyranine fluorescence might find useful applications in chronic dermatologic diseases with an impaired skin barrier, such as in atopic dermatitis, especially in following the response to therapy. Finally, comedones on the face of acne patients also stain brightly, and the benefit of comedolytic agents could be assessed by their steady disappearance during treatment (unpublished observations). REFERENCES (1) K. P. Wilhelm, C. Surber, and H. I. Maibach, Quantification of sodium lauryl sulphate dermatitis in man: Comparison of four techniques: skin color reflectance, transepidermal water loss, laser Doppler flow measurement and visual score, Arch. Dermatol. Res., 281, 293-295 (1989). (2) T. Agner and J. Serup, Sodium lauryl sulphate for irritant patch testing--A dose-response study using bioengineering methods for determination of skin irritation,J. Invest. DermatoL, 95,543-547 (1990). (3) S. Seidenari and A. Di Nardo, B scanning evaluation of irritant reactions with binary transformation and image analysis, Acta Derre. Venereol., S175, 9-13 (1992). (4) R. A. Tupker, C. Willis, E. Berardesca, C. H. Lee, M. Fartasch, T. Agner, and J. Serup, Guidelines on sodium lauryl sulfate (SLS) exposure tests. A report from the standardization group of the European Society of Contact Dermatitis, Contact Dermatitis, 37, 53-69 (1997). (5) S. Seidenari and B. Belletti, Instrumental evaluation of subclinical irritation induced by sodium lauryl sulfate, Contact Dermatitis, 30, 175 (1994). (6) J. Pinnagoda, R. A. Tupker, T. Agner, and J. Setup, Guidelines for transepidermal water loss (TEWL) measurements. A report from the standardization group of the European Environmental and Contact Dermatitis Society, Contact Dermatitis, 22, 164-178 (1990). (7) L. H. Jansen, M. T. Hojyo-Tomoko, and A.M. Kligman, Improved fluorescence staining technique for estimating turnover of the human stratum comeurn, Br. J. Dermatol., 90, 9-12 (1974). (8) K. P. Wilhelm, J. C. Saunders, and H. I. Maibach, Increased stratum corneum turnover induced by subclinical irritant dermatitis, Br. J. Dermatol. 122, 793-798 (1990). (9) M. Paye, F. A. Simion, and G. E. Pierard, Dansyl chloride labelling of stratum comeurn: Its rapid extraction from skin can predict skin irritation due to surfactants and cleansing products, Contact Dermatitis, 30, 91-96 (1994). (10) G. E. Piefatal, Microscopic evaluation of the dansyl chloride test, Dermatology, 185, 37-40 (1992). (11) S. Seidenari, B. Belletti, and G. Pellacani, Time course of skin changes induced by short-term occlusion with water: Evaluation by TEWL, capacitance and B-scanning echography, Skin Res. Technol., 2, 52-53 (1996).

j. Cosmet. Sci., 49, 39-67 (January/February 1998) Preprints of the 1997 Annual Scientific Seminar December 4-5, 1997 New York Hilton New York, NY 39