JOURNAL OF COSMETIC SCIENCE 418 From the additional experiments, the CUFO emulsion had a minimum droplet size (173.73 nm ± 0.43 nm) when HLB = 14.90 in Figure 15A and 127.13 nm ± 95.45 nm for the HLB value of 14.75 in Figure 15B. The CUPO emulsion had a minimum droplet size at an HLB value of 15.35 (126.73 nm ± 0.76 nm) (Figure 15A) and an HLB value of 15.40 (106.27 nm ± 4.89 nm) (Figure 15B). To confi rm wh ether the additional experiments using polysorbate 20 showed the same tendency as the previous experiments, the experiments were conducted using Figure 8. Turbidities of CUFO and CUPO emulsions with (A) surfactant (polysorbate 60: polysorbate 20) HLB range 14.90–16.70, and (B) surfactant (sorbitan sesquioleate: polysorbate 20) HLB range 13.45–16.70, which were measured by a UV-vis spectrophotometer after 1 week storage at room tem- perature (n = 3 ±SD). Figure 7. Turbidities of CUFO, CUPO, HF, and CJSO emulsions in the HLB range from 3.70 to 14.90, which were measured by a UV-vis spectrophotometer after 1 week storage at room temperature (n = 3 ±SD).

DETERMINATION OF REQUIRED HLB VALUE 419 HF and CJSO emulsions. The HF emulsion had a minimum droplet size of 186.17 nm ± 0.32 nm at an HLB value of 6.30, On the other hand, the CJSO emulsion had a minimum droplet size of 188.67 nm ± 0.63 nm at an HLB value of 6.30 (Figure 16). CONCLUSION In t he present study, we used the method of Orafi diya and Oladimeji so that the re- quired HLB value is the HLB value when the emulsion has the maximum turbidity and the minimum droplet size during the storage period (1,2). A series of emu lsions was Figure 9. Turbidities of HF and CJSO emulsions in HLB range 3.70–16.70, which were measured by a UV- vis spectrophotometer after 1 week storage at room temperature (n = 3 ±SD). Figure 10. Turbidity of liquid paraffi n emulsions in HLB range 3.70–14.90 which was measured by a tur- bidity meter after 1 week storage at room temperature (n = 3 ±SD).