DETERMINATION OF REQUIRED HLB VALUE 421 emulsion had the highest turbidity and minimum droplet size at HLB values of 15.35–15.40. To confi rm whet her the additional experiments using polysorbate 20 shows the same tendency as the previous experiments, the experiments were conducted using HF and CJSO emulsions. The HF and CJSO emulsions had the maximum turbidity and the minimum droplet size at an HLB value of 6.30. By measuring th e turbidity [UV-vis spectrophotometer and turbidity meter (Lutron)] and droplet size (particle size analyzer) of each prepared O /W emulsion, fi nally the re- quired HLB values for the CUFO, CUPO, HF, and CJSO were found to lie within 14.75– 14.90, 15.35–15.40, 6.30–7.04, and 5.94–6.30, respectively. Figure 14. Mean droplet size variation of emulsions of HF in the HLB range 3.70–14.90 (n = 3 ±SD). Figure 13. Mean droplet size variations of emulsions of CUFO, CUPO, and CJSO in the HLB range 3.70– 14.90 (n = 3 ±SD).

JOURNAL OF COSMETIC SCIENCE 422 ACKNOWLEDGMENTS This research w as fi nancially supported by the Ministry of Trade, Industry and Energy and the Korea Institute for Advancement of Technology through the National Innovation Cluster R&D program (P0006705_Development of Customized Cosmetic Materials and Formulations). Figure 16. Mean droplet size variations of HF and CJSO emulsions in the HLB range 3.70–16.70 (sorbitan sesquioleate: polysorbate 20) after 1 week storage at room temperature (n = 3 ±SD). Figure 15. Droplet sizes of CUFO and CUPO emulsions with (A) surfactant (polysorbate 60: polysor- bate 20) HLB range 14.90–16.70, and (B) surfactant (sorbitan sesquioleate: polysorbate 20) HLB range 13.45–16.70, which were measured by particle size analyzer after 1 week storage at room temperature (n = 3 ±SD).