DETERMINATION OF REQUIRED HLB VALUE 419 HF and CJSO emulsions. The HF emulsion had a minimum droplet size of 186.17 nm ± 0.32 nm at an HLB value of 6.30, On the other hand, the CJSO emulsion had a minimum droplet size of 188.67 nm ± 0.63 nm at an HLB value of 6.30 (Figure 16). CONCLUSION In t he present study, we used the method of Orafi diya and Oladimeji so that the re- quired HLB value is the HLB value when the emulsion has the maximum turbidity and the minimum droplet size during the storage period (1,2). A series of emu lsions was Figure 9. Turbidities of HF and CJSO emulsions in HLB range 3.70–16.70, which were measured by a UV- vis spectrophotometer after 1 week storage at room temperature (n = 3 ±SD). Figure 10. Turbidity of liquid paraffi n emulsions in HLB range 3.70–14.90 which was measured by a tur- bidity meter after 1 week storage at room temperature (n = 3 ±SD).

JOURNAL OF COSMETIC SCIENCE 420 prepared by using sorbitan sesquioleate (HLB = 3.70) and polysorbate 60 (HLB = 14.90). Evaluation of turbidity using a UV-vis spectrophotometer and a turbidity meter (Lutron) showed a similar tendency. The required HLB values for maximum turbidities of CUFO, CUPO, HF, and CJSO O/W emulsions were measured as 14.90, 14.90, 7.06, and 5.94, respectively. The CUFO and CU PO emulsions had the highest turbidity at the highest HLB value of 14.90. Therefore, CUFO and CUPO needed additional experiments with polysorbate 20 (HLB = 16.70), considering the required HLB value higher than 14.90. From the additional experiments, the CUFO emulsion exhibited the highest turbidity and minimum droplet size at HLB values of 14.75–14.90. The CUPO Figure 12. Mean droplet size variations of emulsions of liquid paraffi n in the HLB range 3.70–14.90 (n = 3 ±SD). Figure 11. Turbidities of CUFO, CUPO, HF, and CJSO emulsions in HLB range 3.70–14.90 which was measured by a turbidity meter after 1 week storage at room temperature (n = 3 ±SD).