Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact Histone H2AX 36 4 4 TJP3 protein 36 4 4 Histone H3 34 4 4 tetratricopeptide repeat domain 21B 30 2 2 Potassium voltage-gated channel subfamily H member 8 29 2 2 Protease serine 1 (Fragment) 26 11 10 x Keratin-associated protein 2-1 25 9 2 TRANSCRIPTION FACTOR 4 25 2 2 LIM domain only protein 7 24 1 1 LIM domain only 7 protein 24 1 1 LIM domain only protein 7 24 1 1 LIM domain only protein 7 24 1 1 Sensor protein 24 1 1 Keratin-associated protein 4-4 22 8 4 Trypsin-2 22 10 9 x Histone H2B type 1-C/E/F/G/I 20 2 2 Protease serine 2 19 11 1 x Trypsin-1 19 11 1 x Protease serine 2 preproprotein 19 11 1 x Prolow-density lipoprotein receptor-related protein 1 19 11 1 Epididymis secretory sperm binding protein Li 134P 19 11 1 Methanethiol oxidase 19 11 1 Histone H3 19 2 1 Histone H3 (Fragment) 19 2 1 Histone H3 B 19 2 1 Histone H3.1 19 2 1 Histone H3.2 19 2 1 Histone H3.3 19 2 1 Histone H3.1t 19 2 1 Histone H3.3 19 2 1 NEDD4-binding protein 1 19 2 1 Epididymis secretory protein Li 50 19 2 1 Ubiquitin C 19 2 1 Ubiquitin-40S ribosomal protein S27a 19 2 1 Helicase/primase complex 19 2 1 Nucleolar protein 8 19 2 1 Nucleolar protein 8 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 DNA polymerase epsilon catalytic subunit A 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 Mitochondrial import inner membrane translocase subunit TIM50 19 2 1 Plexin-B3 19 2 1 LIM domain only protein 7 18 1 1 NOL8 protein 17 2 1 Keratin-associated protein 1-5 16 9 1 Histone H2B 15 2 2 Proliferation marker protein Ki-67 14 2 1 DNA polymerase epsilon catalytic subunit 14 2 1 Appendix 1 Continued JOURNAL OF COSMETIC SCIENCE 264

Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact Splicing factor proline/glutamine-rich protein 14 1 1 Keratin 10 13 5 1 Histone H2B 12 2 2 Histone H3 B 11 2 2 SFPQ protein 11 1 1 Histone H2B 11 2 2 Histone H2B type 2-E 11 2 2 Keratin-associated protein 1-3 10 11 2 Keratin-associated protein 4-7 10 11 2 Histone H2B (Fragment) 10 2 2 Histone H2B type 1-B 10 2 2 Histone H2B type 1-J 10 2 2 Histone H2B type 3-B 10 2 2 Histone H2B type 1-O 10 2 2 Keratin-associated protein 13-1 10 2 2 NPC1-like intracellular cholesterol transporter 1 10 1 1 TIGHT JUNCTION PROTEIN ZO-1 9 5 2 Keratin 1 9 4 4 Keratin 2 9 4 4 Keratin-associated protein 7-1 9 2 2 LIM domain 7 8 1 1 Splicing factor proline- and glutamine-rich protein 8 1 1 RASGRF1 protein OS 8 1 1 Ubiquitin carboxyl-terminal hydrolase 6 8 1 1 Zinc fi nger protein 40 8 1 1 DNA polymerase theta variant 8 1 1 Polymerase (DNA directed) theta isoform CRA 8 1 1 DNA polymerase theta 8 1 1 DNA polymerase theta 8 1 1 DNA polymerase theta 8 1 1 Uncharacterized protein 8 1 1 Keratin 1 OS=Homo sapiens OX=9606 GN=KRT1 PE=3 SV=1 8 1 1 Histone H2B type F-S 7 2 2 Keratin 34 7 1 1 Fibronectin type-III domain-containing protein 3A 7 1 1 Kinectin 1 6 1 1 Trinucleotide repeat-containing gene 6B protein 6 1 1 Microtubule-associated serine/threonine-protein kinase 4 6 1 1 Keratin 1 5 1 1 Uncharacterized protein (Fragment) 4 1 1 Histone H2B type 1-N 4 2 2 FAST kinase domain-containing protein 1 4 1 1 NPC1-like intracellular cholesterol transporter 1 4 1 1 Spectrin-like protein 3 1 1 Nesprin-1 3 1 1 Zinc fi nger homeobox protein 4 3 1 1 Zinc fi nger homeobox protein 4 3 1 1 Zinc fi nger homeodomain 4 3 1 1 DNA-directed RNA polymerase III subunit RPC2 3 1 1 Appendix 1 Continued HAIR SHAPE PROTEOMICS 265