DETERMINATION OF CHLORINATED PHENOLS If the ratio of the E• values at 33.8 and 35.8 Kcm -• was less than 2.15, chloroxylenols were absent and the hexachlorophene content was cal- culated from the equation-- s H % w/w hexachlorophene = 100 x E•.s/E•.s where s H • E33.0 and the respective E• values of the sample and E3•. o are hexachlorophene at 33.8 Kcm-L However, if this was not the case the following simultaneous equations were solved for the hexachlorophene and chloroxylenol contents. s x H = E3.0(x) + (h) s x H and E•s.s = E•s.8 (x) q- E3s.8 (h) where s • E•s.8 is the E• value of the sample at 35.8 Kcm -•, E•X•.8 and x are the E• values of the chloroxylenol at 33.8 and 35.8 Kcm -• respectively, H • E•s.s is the E• value of hexachlorophene at 35.8 Kcm -• and (x) and (h) are the respective concentrations of chloroxylenol and hexachlorophene. RESULTS AND DISCUSSION o, \ ,,,/ , o \\•'-•'1'•1 '111 h,•..•_ WAVELENGTH Kcr• [ Figure 1 Spectra of hexachlorophene (a) and 4-chloro 3:5-dimethylphenol (b) in N/10 methanolic HC1. Fig. 1 shows the absorption spectra of hexachlorophene and

JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS chloroxylenol (4-chloro-3:5-dimethylphenol) in N/10 methanolic HC1 solvent. The former showed an absorption maxima at 33.8 Kcm -• while the maxima for the latter occurred at 35.8 Kcm-L It was found that the maxima for other chloroxylenols occurred close to this value, the actual range being 35.5 to 35.9 Kcm -• (281.5 to 279 m•). the E} values for various chlorinated phenols at 33.8 and 35.8 Kcm -• in N/10 methanolic HC1 are recorded in Table I. Table I E, x values at 33.8 and 35.8 Kcm -x for chlorinated phenols in N/10 methanolic HC1. Phenol Hexachlorophene 4-Chloro-3:5-dimethyl 4-Chloro-2:3-dimethyl 4-Chloro-2:5~dimethyl 4-Chloro-2:6-dimethyl 2-Chloro-3:5-dimethyl 6-Chloro-3:4-dimethyl El value at 33.8 Kcm -x 138.4 4.05 7.7 20.2 3.2 2.72 21.7 at 35.8 Kcm -x 63.8 95.5 101.0 138.8 98.8 103.5 166.0 Spectrum (a) in Fig. 2 is of the N/10 methanolic HC1 extract of a powdered deodorant formulated to contain 0.5% w/w hexachlorophene and 0.12% w/w 4-chloro-3:5-dimethylphenol. In spectrum (b), which was the extract of a product formulated to contain hexachlorophene, the decreased absorption at 35.8 Kcm -• confirmed the absence of a chloroxy- lenol. From a series of replicate determinations performed on a powdered deodorant, formulated to contain 0.5% w/w hexachlorophene, the standard deviation for the determination of hexachlorophene was found to be 4-0.005%. The results obtained for a powdered deodorant formulated to contain 0.5% w/w hexachlorophene and 0.12% 4-chloro-3:5-dimethyl- phenol are recorded in Table II. Table II Hexachlorophene and 4-chloro-3:5-dimethylphenol content of powdered deodorant Sample No. % w/w hexachlorophene % w/w 4-chloro-3:5-dimethyl phenol 0.472 0.486 0.480 0.487 0.479 0.117 0.119 0.115 0.125 0.120