ANTI-AGING COMPOUNDS FROM JEJU ISLAND 29
Table III
Elastase Inhibition Activity of Plant Extracts
Inhibition (%)
Plant: Botanical name
(part used)" 100 µg/ml 50 µg/ml 10 µg/ml IC50 (µg/ml)6
Acanthopanax koreanum (LF)
Acanthopanax koreanum (RT) 9.2 5.2
Acanthopanax koreanum (ST) 1.3 3.5
Aruncus aethusifolius (RT) 6.7 4.1 8.7
Aster spathulifolius (LT) 17.8 20.1 19.4
Aster spathulifolius (RT) 25.5 26.2 26
Brassica campestris (ST) 13 12.6 17.4
Brassica campestris (FL) 15.6 23.5 21.2
Brassica campestris (RT) 15.5 13 15.4
Camellia japonica (LF) 45.2 33.1 14.3 124.6
Camellia sinensis (SD) 14.9 17.1 17
Capsicum annum (WP) 61.7 41.4 14.7 71.1
Cassia nomame (WP) 29.9 22.8 10.7
Chrysanthemum indicum (WP) 2.3 3.5
Chrysanthemum zawadskii (WP) 11.4 10.4 10.1
C imicifi,ga acerina (RT) 6.9 1.1 4
Citrus grandis (LF) 13.9 12.5 12
Citrus grandis (ST) 5.7 8.6 7.3
Citrus unshiu (LF) 3.7 22.9
Citrus unshiu (ST) 7.1
C Zematis terniflora (LT) 8.8 11 10.5
Clerodendron trichotomum (LF) 2.5
Clerodendron trichotomum (ST) 1.5 1.1
C ommelina communis L. (WP) 8.2 0.6 3
Crinum asiaticum (SD) 0.4
Crinum asiaticum (LT) 10 15.7 18.8
Crinum asiaticum (RT) 14.2 16.3 22.1
Daphniphyllum macropodum (LF) 5.9 11.8
Daphniphyllum macropodum (ST) 3.7 13.2
Daucus carota L. (LF) 3.9 7.9 7.3
Elsholtzia splendens (WP) 1.6 6.5 4.5
Erigeron annuus (LT) 0.4
Erigeron annuus (RT)
Euphorbia esula L. (WP) 14.5 15 15.1
Euphorbia helioscopia (WP) 40.4 36.7 22.3
Euphorbia jolkini (SD) 14.7 12.6 3.5
Euphorbia jolkini (WP) 7.3 15.1 39.4
Farfugium japonicum (WP) 1.8
F ilipendula glaberrima (RT) 52.8 40.8 22.6 88.2
Hizikia fusiformis (WP) 30.2 30.1 31.9
Kummerowiastriata (WP) 5.4
Lycoris aurea (RT) 6.4 17.3 18.9
Maackia fauriei (ST) 2.5 10 4.9
Miscanthus sinensis (FL) 20 17 12.8
Miscanthus sinensis (RT) 10.8 7.6 8.8
Miscanthus sinensis (ST) 12.9 15.8 11.9
Myriophyllum Jpicatum L. (WP) 19.7 19 12.7
Narcissus tazetta (LT) 15.4 15.2 14.8
Nymphaea tetrar,ona (RH) 54.7 46.7 31.2 70.4
Persicaria hydropiper (WP) 60.4 52.9 15.8 46.7
(continued)

30 JOURNAL OF COSMETIC SCIENCE
Table III (continued)
DPPH radical scavenging activity (%)
Plant: Botanical name
(part used? 100 µg/ml 50 µg/ml 10 µg/ml IC50 (µg/ml)
Potamogeton crispus L. (WP)
Pyrrosia hastata (LT)
Pyrrosia hastata (RT)
Sasa quelpaertensis (LT)
Scirpus tabernaemontani (LT)
Solanum nigrum L. (WP)
Solanum tuberosum L. (RH)
Thymus quinquecostatus (WP)
T orreya nucifera (LF)
T orreya nucifera (ST)
Typha orientalis (SD)
Zingiber mioga (ST)
6.5
8.4
48.5
24.9
28.1
6.9
40.4
9.5
8.6
1.8
55.7
13.3
5.1 6.4
1.8 6
31.5 11.23 105.l
25.2 25
22.2 11.3
8.5 6.3
38 34.9
15 .1 17.7
7.9 6.5
4 3.7
48.5 33.3 60.1
14.9 10.3
a Part used: LF (leaf), ST (stem), LT (leaves and twigs), RT (root), WP (whole plant), SD (seed), FL (flower),
RH (rhizome).
6 IC50: Concentration (µg/ml) at which the inhibition %of elastase activity is 50%.
140
lJJ
lOJ
-� 8)
]-�
t)
40
0
Control TO PHA PHY FG CA NT CJ EGCG
Figure 3. Cytotoxicity in human fibroblasts (ATCC, CRL-2076). All plant extracts were used at 100
µg/ml, except EGCG (25 µM). TO: Typha orientalis (seed). PHA: Pyrrosia hastata (root). PHY: Persicaria
hydropiper (whole plant). FG: Filipendula glaberrima (root). CA: Capsicum annum (whole plant). NT: Nymphaea
tetragona (rhizome). CJ: Camellia japonica (leaf). EGCG: (-)-epigallocatechin-3-gallate.
CONCLUSIONS
Jeju Island is the largest island located in the southernmost part of the Republic of
Korea. Mt. Halla rises 1950 meters above sea level in the center of the island. Therefore,
due to its altitude and latitude, a variety of plant species are present on the island in spite