30 JOURNAL OF COSMETIC SCIENCE
Table III (continued)
DPPH radical scavenging activity (%)
Plant: Botanical name
(part used? 100 µg/ml 50 µg/ml 10 µg/ml IC50 (µg/ml)
Potamogeton crispus L. (WP)
Pyrrosia hastata (LT)
Pyrrosia hastata (RT)
Sasa quelpaertensis (LT)
Scirpus tabernaemontani (LT)
Solanum nigrum L. (WP)
Solanum tuberosum L. (RH)
Thymus quinquecostatus (WP)
T orreya nucifera (LF)
T orreya nucifera (ST)
Typha orientalis (SD)
Zingiber mioga (ST)
6.5
8.4
48.5
24.9
28.1
6.9
40.4
9.5
8.6
1.8
55.7
13.3
5.1 6.4
1.8 6
31.5 11.23 105.l
25.2 25
22.2 11.3
8.5 6.3
38 34.9
15 .1 17.7
7.9 6.5
4 3.7
48.5 33.3 60.1
14.9 10.3
a Part used: LF (leaf), ST (stem), LT (leaves and twigs), RT (root), WP (whole plant), SD (seed), FL (flower),
RH (rhizome).
6 IC50: Concentration (µg/ml) at which the inhibition %of elastase activity is 50%.
140
lJJ
lOJ
-� 8)
]-�
t)
40
0
Control TO PHA PHY FG CA NT CJ EGCG
Figure 3. Cytotoxicity in human fibroblasts (ATCC, CRL-2076). All plant extracts were used at 100
µg/ml, except EGCG (25 µM). TO: Typha orientalis (seed). PHA: Pyrrosia hastata (root). PHY: Persicaria
hydropiper (whole plant). FG: Filipendula glaberrima (root). CA: Capsicum annum (whole plant). NT: Nymphaea
tetragona (rhizome). CJ: Camellia japonica (leaf). EGCG: (-)-epigallocatechin-3-gallate.
CONCLUSIONS
Jeju Island is the largest island located in the southernmost part of the Republic of
Korea. Mt. Halla rises 1950 meters above sea level in the center of the island. Therefore,
due to its altitude and latitude, a variety of plant species are present on the island in spite

(A)
MMP-
(B)
100
OJ 8)
0:::
·vi
BJ
.40
0:::
JJ
ANTI-AGING COMPOUNDS FROM JEJU ISLAND
NcmSNP SNP TO PHA PHY FG CA NT CJ EGCG
31
Figure 4. The expression of MMP-1 in human fibroblasts (ATCC, CRL-207 6). All plant extracts were used
at 100 µg/ml, except EGCG (25 µM). Non-SNP: in the absence of sodium nitroprusside. SNP: in the
presence of sodium nitroprusside. TO: Typha orientalis (seed). PHA: Pyrrosia hastata (root). PHY: Persicaria
hydropiper (whole plant). FG: Filipendula glaberrima (root). CA: Capsicum annum (whole plant). NT: Nymphaea
tetragona (rhizome). CJ: Camellia japonica (leaf). EGCG: (-)epigallocatechin-3-gallate.
of its small area. We searched for plants with effective anti-aging compounds for the skin
among the natural plants of Jeju Island.
Sixty plants from Jeju Island were extracted, and their activities were investigated for
free radical scavenging activity, elastase inhibition activity, and reduction of the expres-
sion of MMP-1 mRNA related to collagenase, which is known to degrade collagenase
and induce wrinkling of the skin. As a result, we found twelve plants with highly effective
DPPH radical scavenging activity and seven plants with good anti-elastase activity. Five
of the seven species with superior effects on elastase activity inhibition also had high
DPPH radical scavenging activity, which suggests that there is a certain correlation
between the causes of the two activities. When comparing the inhibition activities of the
seven extracts on MMP-1 expression in human fibroblasts with EGCG as a positive
control, four extracts, Persicaria hydropiper (whole plant), Filipendula glaberrima (root),