JOURNAL OF COSMETIC SCIENCE 388 (27) L. W. Soromou, N. Chen, L. Jiang, M. Huo, M. Wei, X. Chu, F. M. Millimouno, H. Feng, Y. Sidime, and X. Deng, Astragalin attenuates lipopolysaccharide-induced infl ammatory responses by down-regulating NF-κB signaling pathway, Biochem. Biophys. Res. Commun., 419, 256–261 (2012). (28) S. Deng, Z. Deng, Y. Fan, Y. Peng, J. Li, D. Xiong, and R. Liu, Isolation and purifi cation of three fl a- vonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chroma- tography, J. Chromatogr. B. Analyt. Technol. Biomed. Life. Sci., 877, 2487–2492 (2009). (29) E. Ohkoshi, H. Miyazaki, K. Shindo, H. Watanabe, A. Yoshida, and H. Yajima, Constituents from the leaves of Nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice, Planta Med., 73, 1255– 1259 (2007). (30) S. Nakamura, S. Nakashima, G. Tanabe, Y. Oda, N. Yokota, K. Fujimoto, T. Matsumoto, R. Sakuma, T. Ohta, K. Ogawa, S. Nishida, H. Miki, H. Matsuda, O. Muraoka, and M. Yoshikawa, Alkaloid con- stituents from fl ower buds and leaves of sacred lotus (Nelumbo nucifera, Nymphaeaceae) with melanogen- esis inhibitory activity in B16 melanoma cells, Bioorg. Med. Chem., 21, 779–787 (2013). (31) Y. B. Wu, L. J. Zheng, J. G. Wu, T. Q. Chen, J. Yi, and J. Z. Wu, Antioxidant activities of extract and fractions from receptaculum nelumbinis and related fl avonol glycosides, Int. J. Mol. Sci., 13, 7163–7173 (2012).

J. Cosmet. Sci., 65, 389–401 (November/December 2014) 389 Trophic effect of a methanol yeast extract on 3T3 fi broblast cells DOMINIQUE GALLO, MONIQUE DILLEMANS, DAVID ALLARDIN, FABIAN PRIEM, LAURENCE VAN NEDERVELDE, Commission communautaire française (Cocof), Service des Industries Biochimiques, Institut Meurice, 1070 Brussels, Belgium (D.G., M.D., D.A., L.V.N.), Commission communautaire française (Cocof), Département des Substances Naturelles et de Biochimie, Institut Meurice, 1070 Brussels, Belgium (D.G.), Eleonor sprl, 1310 La Hulpe, Belgium (F.P.). Accepted for publication October 20, 2014. Synopsis With regard to the increase of human life expectancy, interest for topical treatments aimed to counteract skin aging is still growing. Hence, research for bioactive compounds able to stimulate skin fi broblast activity is an attractive topic. Having previously described the effects of a new methanol yeast extract on growth and metabolic activity of Saccharomyces cerevisiae, we studied its effects on 3T3 fi broblasts to evaluate its potential antiaging property. This investigation demonstrates that this extract increases proliferation as well as migra- tion of 3T3 cells and decreases their entrance in senescence and apoptosis phases. Altogether, these results open new perspectives for the formulation of innovative antiaging topical treatments. INTRODUCTION Skin is an organ the function of which is mainly to protect an organism against the external environment. In one way, skin impedes physical, chemical, and microbiologi- cal hazards and in the other, it regulates loss of water, electrolytes, and heat (1). More- over, known to contain a large number of Langerhans cells, skin is considered a real sentry with regard to body immunity (2). In addition, the extensive nerve network present in skin makes an effective sensory link between organism and its environment. Epidermis, dermis, and hypodermis are the three main layers of the skin. It should be stressed that these layers derive from different embryonic origins. While epidermis derives from ectoderm, dermis and hypodermis are both of mesodermal origin. Thus, by contrast to epidermis, which is mostly composed of epithelial cells, dermis and hy- podermis are mainly composed of mesenchymal cells (i.e., fi broblasts and adiposities, respectively). Address all correspondence to Monique Dillemans at mdillemans@hotmail.fr.