JOURNAL OF COSMETIC SCIENCE 62 product was used until 8 weeks after the treatment (Table III). Conversely, the test cream produced a statistically signifi cant decrease in eye wrinkles after 4 weeks of use. In addi- tion, the results show that there were signifi cant differences between the test and placebo creams in Rz and Rt parameters after 8 weeks of treatment. Our fi ndings indicate that the test cream smoothed the skin surface and reduced wrinkles. CONCLUSIONS This study was carried out to evaluate the antiaging effect of PT-FX50. The results show that PT-FX50 is not cytotoxic at concentrations of d20 ppm. We have also shown that PT-FX50 decreases the expression levels of MMP-1, MMP-2, and MMP-9 in HDFN cells. Furthermore, the fucoxanthin-containing cream we prepared showed considerable effi cacy as it signifi cantly increased skin moisture and elasticity after 4 weeks of treat- ment. These results demonstrate that the test product has excellent antiwrinkle and moisturizing effects. Furthermore, our fi ndings suggest that fucoxanthin can be used as a functional cosmetic agent. A CKNOWLEDGMENT T his research was a part of the project titled “Development and industrialization of high value cosmetic raw materials from marine microalgae,” funded by the Ministry of Oceans and Fisheries, Korea. R EFERENCES ( 1) M. A. Farage, K. W. Miller, P. Elsner, and H. I. Maibach, Intrinsic and extrinsic factors in skin ageing: a review, Int. J. Cosmet. Sci., 30, 87–95 (2008). ( 2) H. K. Biesalski, M. Berneburg, T. Grune, M. Kerscher, J. Krutmann, W. Raab, J. Reimann, T. Reuther, L. Robert, and T. Schwarz, Hohenheimer Consensus Talk. Oxidative and premature skin ageing, Exp. Dermatol., 12(Suppl. 3), 3–15 (2003). Table III Evaluation of Skin Wrinkles Parameters Day 0 Day 28 Day 56 Rab Test 5.295 ± 0.920 5.062 ± 0.980 5.016 ± 0.960 Placebo 5.258 ± 1.053 5.209 ± 1.005 5.105 ± 0.834 p valuea — 0.140 0.413 Rzc Test 32.871 ± 6.221 30.507 ± 4.655 28.633 ± 4.701 Placebo 31.499 ± 5.479 30.690 ± 5.883 30.056 ± 4.093 p value — 0.149 0.034 Rtd Test 46.110 ± 8.781 41.578 ± 6.516 38.630 ± 5.569 Placebo 44.183 ± 7.821 42.935 ± 9.084 41.356 ± 4.807 p value — 0.123 0.037 a p value: signifi cant probability, linear mixed model, p 0.05 when test and placebo products are compared. b Ra: arithmetic average roughness. c Rz: arithmetic average of the difference segment roughness calculated from fi ve succeeding measurements. d Rt: the distance between the highest mountain and the lowest value.

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