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J. Cosmet. Sci., 71, 367–375 (November/December 2020) 367 Detection and Analysis of Ceramide in Skin and Blood in a Healthy Chinese Population CONG XIN, YAOCHI WANG, YULING CHANG, BO ZHANG, and SEN YANG , Institute of Dermatology and Department of Dermatology, the First Affi liated Hospital, Anhui Medical University, Hefei, China (C.X., S.Y.), Clinic Medical College of Anhui Medical University, Hefei, China (Y.W.), Department of Dermatology, the People’s Hospital of Zhengzhou, Zhengzhou, China (Y.C.), Wuxi Branch of Zhongda Hospital, Southeast University, Wuxi, China (S.C.), Department of Oncology, the Second Affi liated Hospital, Anhui Medical University, Hefei, China (B.Z.) Accepted for publication April 12, 2020. Synopsis To explore the signifi cance of ceramide in the skin barrier and its potential utility within the cosmetics industry, an accurate and high-speed method was used to detect the types of ceramides in the skin and blood of a healthy Chinese population. Forearm cortical skin stratum corneum samples were obtained from four healthy subjects using a noninvasive method. In addition, these subjects were collected intravenously to obtain blood samples. Ceramides were detected in skin and blood samples using high-performance liquid chromatography coupled with specialized high-resolution Fourier Transform mass spectrometry machine. Data were analyzed using full-fl ow lipid analysis software. Peaks representing ceramides were detected in all skin samples and some blood samples. The results show that ceramides in skin are predominantly long-chain ceramides, but mainly short-chain in the blood. Simple and fast qualitative and quantitative analysis of ceramide in the skin and blood provides a basis for the precise addition of ceramide in future skin care products and the metabolic regulation and prevention of various diseases. INTRODUCTION The stratum corneum (SC) of the epidermis is the main barrier to prevent the entry of external substances into the human body and reduce trans-epidermal water loss (1). Intercellular lipids mainly include ceramide (about 50%), cholesterol (about 25%), free fatty acids (about 15%), and a small amount of phospholipids. Ceramides combined with cornifi ed envelope have a physiological function in corneocyte fl exibility (2). Ceramide is closely related to many diseases. Decreased ceramide content in the SC will destroy the skin barrier, including atopic dermatitis (AD), psoriasis, and other diseases (3). Address all correspondence to Sen Yang at yang2004sen@163.com and Bo Zhang at alvinbo@163.com . Cong Xin and Yaochi Wang contributed equally to this work.