Study Parameters measured Method Measurement method Skin area studied Time, w C. asiatica product Placebo/ comparatorp AEs/dropouts Tangsumroengwongwong (20) Wrinkles Digital camera Samsung NX100 Rao-goldman Five point visual Scoring Scale 15 mm below lower eyelid 0 3.19 ± 0.53 3.28 ± 0.58 0.5 48-h forearm patch test 4 3.19 ± 0.53 3.28 ± 0.58 0.5 8 3.06 ± 0.61 3.16 ± 0.67 0.5 No reactions 12 2.91 ± 0.64 2.91 ± 0.53 0.9 15 mm lateral from lateral canthus 0 3.38 ± 0.61 3.38 ± 0.60 1.00 CAext =10 4 3.38 ± 0.61 3.38 ± 0.61 1.00 8 3.22 ± 0.66 3.16 ± 0.62 0.67 Tretinoin = 35 rash, scaling, burning 12 3.13 ± 0.71 3.06 ± 0.71 0.72 Visioscan VC98 Skin wrinkle 15 mm below lower eyelid 0 50.5 ± 5.1 50.7 ± 5.3 0.92 Three dropouts reason NR 4 50.4 ± 5.0 50.7 ± 5.2 0.90 8 49.2 ± 5.3 49.8 ± 5.4 0.53 12 48.0 ± 5.0 48.0 ± 5.1 0.94 15 mm lateral from lateral canthus 0 53.3 ± 4.8 53.5 ± 4.4 0.7 4 53.3 ± 4.9 53.5 ± 4.5 0.9 8 51.9 ± 4.4 52.0 ± 4.3 0.9 12 51.0 ± 4.7 51.1 ± 4.6 0.7 Moisture Corneometer Skin resonant frequency, kHz 15 mm below lower eyelid 0 47.2 ± 3.2 45.2 ± 3.7 0.05 4 52.1 ± 2.9 45.2 ± 3.7 0.001 8 56.1 ± 3.1 45.6 ± 3.8 0.001 12 59.7 ± 3.8 45.6 ± 3.7 0.001 15 mm lateral from lateral canthus 0 47.2 ± 4.3 47.0 ± 4.6 0.9 4 49.5 ± 3.9 46.7 ± 4.7 0.003 8 51.2 ± 3.7 46.7 ± 4.9 0.001 12 53.1 ± 3.7 46.7 ± 4.9 0.001 Satisfaction 12 No quantitative data AEs Visual observation Scarlet, dry peeling skin, or stinging skin Either site 4 N = 5 N = 18 8 N = 3 N = 12 12 N = 2 N = 5 Table III Continued JOURNAL OF COSMETIC SCIENCE 446

Study Parameters measured Method Measurement method Skin area studied Time, w C. asiatica product Placebo/ comparatorp AEs/dropouts Tongsrikeaw (21) Wrinkle Digital camera Rao-goldman 15 mm lateral from lateral canthus 0 3.48 ± 0.55 3.48 ± 0.55 ns 0 4 3.48 ± 0.55 3.48 ± 0.55 ns Five-point visual score 8 3.42 ± 0.55 3.44 ± 0.54 0.7 12 3.38 ± 0.53 3.42 ± 0.54 0.5 Measured at w 12 15 mm below lower eyelid 0 3.36 ± 0.55 3.36 ± 0.55 NS 4 3.36 ± 0.55 3.36 ± 0.55 NS 8 3.32 ± 0.55 3.34 ± 0.54 0.6 12 3.30 ± 0.54 3.32 ± 0.54 0.6 Visioscan VC98 Skin wrinkles 15 mm lateral from lateral canthus 0 58.6 ± 6.6 58.3 ± 6.0 0.6 4 56.8 ± 5.9 56.6 ± 5.9 0.7 8 54.1 ± 5.9 53.9 ± 5.9 0.7 12 51.9 ± 5.9 51.4 ± 6.2 0.5 15 mm below lower eyelid 0 56.2 ± 7.1 54.9 ± 6.9 0.1 4 54.1.±6.9 53.3 ± 6.7 0.2 8 50.2 ± 6.2 49.7 ± 6.7 0.002 12 47.1 ± 5.6 46.6 ± 6.6 0.5 Satisfaction Patient satisfaction questionnaire Patient satisfaction score 15 mm from lateral canthus. 15 mm below lower eyelid 12 3.52 ± 1.05 3.36 ± 0.99 NA AEs Visual observation Either site 4 No AE No AE 8 No AE No AE 12 No AE No AE Table III Continued EFFICACY AND SAFETY OF CENTELLA ASIATICA 447