Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact Splicing factor proline/glutamine-rich protein 14 1 1 Keratin 10 13 5 1 Histone H2B 12 2 2 Histone H3 B 11 2 2 SFPQ protein 11 1 1 Histone H2B 11 2 2 Histone H2B type 2-E 11 2 2 Keratin-associated protein 1-3 10 11 2 Keratin-associated protein 4-7 10 11 2 Histone H2B (Fragment) 10 2 2 Histone H2B type 1-B 10 2 2 Histone H2B type 1-J 10 2 2 Histone H2B type 3-B 10 2 2 Histone H2B type 1-O 10 2 2 Keratin-associated protein 13-1 10 2 2 NPC1-like intracellular cholesterol transporter 1 10 1 1 TIGHT JUNCTION PROTEIN ZO-1 9 5 2 Keratin 1 9 4 4 Keratin 2 9 4 4 Keratin-associated protein 7-1 9 2 2 LIM domain 7 8 1 1 Splicing factor proline- and glutamine-rich protein 8 1 1 RASGRF1 protein OS 8 1 1 Ubiquitin carboxyl-terminal hydrolase 6 8 1 1 Zinc fi nger protein 40 8 1 1 DNA polymerase theta variant 8 1 1 Polymerase (DNA directed) theta isoform CRA 8 1 1 DNA polymerase theta 8 1 1 DNA polymerase theta 8 1 1 DNA polymerase theta 8 1 1 Uncharacterized protein 8 1 1 Keratin 1 OS=Homo sapiens OX=9606 GN=KRT1 PE=3 SV=1 8 1 1 Histone H2B type F-S 7 2 2 Keratin 34 7 1 1 Fibronectin type-III domain-containing protein 3A 7 1 1 Kinectin 1 6 1 1 Trinucleotide repeat-containing gene 6B protein 6 1 1 Microtubule-associated serine/threonine-protein kinase 4 6 1 1 Keratin 1 5 1 1 Uncharacterized protein (Fragment) 4 1 1 Histone H2B type 1-N 4 2 2 FAST kinase domain-containing protein 1 4 1 1 NPC1-like intracellular cholesterol transporter 1 4 1 1 Spectrin-like protein 3 1 1 Nesprin-1 3 1 1 Zinc fi nger homeobox protein 4 3 1 1 Zinc fi nger homeobox protein 4 3 1 1 Zinc fi nger homeodomain 4 3 1 1 DNA-directed RNA polymerase III subunit RPC2 3 1 1 Appendix 1 Continued HAIR SHAPE PROTEOMICS 265

Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact DNA-directed RNA polymerase subunit beta 3 1 1 BTB/POZ domain-containing protein 8 3 2 2 Fibronectin type III domain containing 3A 3 2 2 Conserved oligomeric Golgi complex component 1 3 1 1 Histone H3 2 1 1 Histone H2B 2 1 1 Histone H2B type 1-K 2 1 1 Histone H2B 2 1 1 KRT83 protein 2 1 1 Nesprin-1 2 1 1 Nesprin-1 2 1 1 Kinectin 2 1 1 FLJ20980 fi s clone 2 1 1 Receptor protein-tyrosine kinase 2 1 1 NPC1-like intracellular cholesterol transporter 1 2 1 1 Keratin 1 (Fragment) 2 1 1 CTD small phosphatase-like protein 2 2 1 1 CTD small phosphatase-like protein 2 2 1 1 Coiled-coil domain-containing protein 129 2 1 1 Keratin 1 1 1 1 Keratin 1 1 1 1 Histone H2B 1 2 2 Histone H2B type 1-D 1 2 2 EPB41 protein 1 1 1 Protein 4.1 1 1 1 Probable ribonuclease ZC3H12B 1 1 1 Zinc fi nger CW-type PWWP domain protein 2 1 1 1 HCTP4 1 1 1 Keratin-associated protein 4-9 1 1 1 Ryanodine receptor 3 1 1 1 Ryanodine receptor 3 1 1 1 Ryanodine receptor 3 1 1 1 Ryanodine receptor 3 1 1 1 Cell division cycle-associated protein 2 1 1 1 cDNA FLJ78763 1 1 1 RNA binding motif protein 10 isoform 2 1 1 1 FAST kinase domain-containing protein 1 1 1 1 Keratin-associated protein 9-2 1 1 1 Neurexin-3-beta 1 1 1 Neurexin-3 1 1 1 Neurexin-3-beta 1 1 1 1-phosphatidylinositol 4 5-bisphosphate phosphodiesterase gamma-1 1 1 1 Keratin 40 1 1 1 T-cell antigen CD7 1 1 1 ATP-binding cassette sub-family A member 5 1 1 1 ATP-binding cassette protein 1 1 1 Nipped-B-like protein 1 1 1 Nipped-B protein 1 1 1 MX2 1 1 1 Interferon-induced GTP-binding protein 1 1 1 Appendix 1 Continued JOURNAL OF COSMETIC SCIENCE 266