Appendix 1 Overview of the 217 proteins identifi ed and quantifi ed in at least 75% of the samples. Proteins (but not protein groups) are listed with the obtained sequence coverage, number of peptides identifi ed for that protein, number of unique peptides identifi ed per protein. Additionally, some proteins can be linekd to the proteomic procedure used and are indicated with an ‘x’ as artefact. Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact Keratin-associated protein 2-3 99 41 28 Keratin 36 97 44 4 Keratin type I cytoskeletal 39 96 27 3 Keratin-associated protein 3-1 96 26 2 Keratin-associated protein 4-2 96 26 2 Keratin 81 93 105 1 Keratin 83 93 100 5 Keratin 85 92 63 15 Keratin 85 91 96 8 Keratin 33B 90 82 16 Keratin 32 90 57 12 Keratin-associated protein 2-4 90 43 5 Keratin 33A 88 68 17 Keratin-associated protein 9-9 87 41 2 Keratin 31 85 88 1 Keratin 38 84 37 5 Histone H4 L 84 22 20 Keratin-associated protein 9-8 83 55 1 Keratin-associated protein 4-6 83 25 1 Keratin-associated protein 11-1 82 23 13 Keratin-associated protein 1-1 81 35 4 Keratin-associated protein 9-6 80 31 1 Keratin-associated protein 13-2 78 15 3 Keratin-associated protein 9-3 75 39 1 Trypsin I (Fragment) 75 15 7 x Keratin associated protein 4-6 73 29 12 cDNA FLJ25625 fi s clone STM02974 72 19 5 PRSS1 protein 71 15 6 x Keratin-associated protein 4-3 70 19 2 Trypsin-1 (Fragment) 69 22 13 x Keratin-associated protein 9-4 68 33 1 Protease serine 1 (Fragment) 68 7 6 x Trypsin-1 67 12 3 x Trypsin-6 66 16 1 x Histone H4 H 62 18 6 Protease serine 1 (Fragment) 62 6 1 x Trypsin-2 62 6 1 x Histone H4 A 57 16 3 V-set and immunoglobulin domain-containing protein 8 57 16 3 Protease serine 1 (Fragment) 56 12 2 x Anionic trypsinogen 56 16 1 x Protein S100-A3 55 19 1 PRSS2 protein (Fragment) 47 6 6 x Protease serine 2 preproprotein 47 6 6 x Protease serine 2 43 9 7 x Keratin-associated protein 3-3 37 22 2 Transcription factor 4 36 4 4 HAIR SHAPE PROTEOMICS 263

Protein Description Sequence Coverage (%) #Peptides #Unique peptides Artefact Histone H2AX 36 4 4 TJP3 protein 36 4 4 Histone H3 34 4 4 tetratricopeptide repeat domain 21B 30 2 2 Potassium voltage-gated channel subfamily H member 8 29 2 2 Protease serine 1 (Fragment) 26 11 10 x Keratin-associated protein 2-1 25 9 2 TRANSCRIPTION FACTOR 4 25 2 2 LIM domain only protein 7 24 1 1 LIM domain only 7 protein 24 1 1 LIM domain only protein 7 24 1 1 LIM domain only protein 7 24 1 1 Sensor protein 24 1 1 Keratin-associated protein 4-4 22 8 4 Trypsin-2 22 10 9 x Histone H2B type 1-C/E/F/G/I 20 2 2 Protease serine 2 19 11 1 x Trypsin-1 19 11 1 x Protease serine 2 preproprotein 19 11 1 x Prolow-density lipoprotein receptor-related protein 1 19 11 1 Epididymis secretory sperm binding protein Li 134P 19 11 1 Methanethiol oxidase 19 11 1 Histone H3 19 2 1 Histone H3 (Fragment) 19 2 1 Histone H3 B 19 2 1 Histone H3.1 19 2 1 Histone H3.2 19 2 1 Histone H3.3 19 2 1 Histone H3.1t 19 2 1 Histone H3.3 19 2 1 NEDD4-binding protein 1 19 2 1 Epididymis secretory protein Li 50 19 2 1 Ubiquitin C 19 2 1 Ubiquitin-40S ribosomal protein S27a 19 2 1 Helicase/primase complex 19 2 1 Nucleolar protein 8 19 2 1 Nucleolar protein 8 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 DNA polymerase epsilon catalytic subunit A 19 2 1 DNA polymerase epsilon catalytic subunit 19 2 1 Mitochondrial import inner membrane translocase subunit TIM50 19 2 1 Plexin-B3 19 2 1 LIM domain only protein 7 18 1 1 NOL8 protein 17 2 1 Keratin-associated protein 1-5 16 9 1 Histone H2B 15 2 2 Proliferation marker protein Ki-67 14 2 1 DNA polymerase epsilon catalytic subunit 14 2 1 Appendix 1 Continued JOURNAL OF COSMETIC SCIENCE 264