488 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS Table I gives a few examples of the wide variety of chemical substances whose separation, purification and identification have been effected by the use of thin-layer chromatographic techniques. Table I Thin-layer chromatographic separations Class of Compound Adsorbent Mobile phase Visualization agent Reference Alkaloids Silica gel Chloroform, Iodoplatinate 115 diethylamine (90 + •o) Antibiotics Silica gel Acetone, Iodine azide 116 methanol (so + 80) Antioxidants Silica gel Chloroform Phosphomolybolic 117 acid Fatty acids Silica gel Petroleum Sulphuric acid 118 ether, diethyl ether (90 q- 10) Food dyes Cellulose n-Propanol, Self indicating 119 ethyl acetate, water, (60 q- 10 q- SO) Pesticides (A) Organo-chlorine (B) Organo-phosphorus (C) Herbicide Plasticizers SteroIs Sugars Vitamins Silica gel Hexane Silver nitrate 85 Silica gel Hexane, Brilliant green and 34 acetone bromine (90 q- 10) Silica gel, Cyclohexane, Silver nitrate 83 kieselguhr benzene, (40 q- 60) acetic acid, liquid paraffin (20 q- 3 q- 2 q- 1) Silica gel Benzene, ethyl Fluorescence 120 acetate (45 q- 5) Silica gel Benzene, ethyl Phosphoric acid 121 acetate (90 q- 10) Alumina, Butanol, Aniline phthalate 122 silica gel acetic acid, (1 q- 1) methanol (20 + 20 + 60) Alumina Benzene o-To]uidine q- KI 123
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