LIGHT SCATTERING IN HAIR CUTICLES 357 a) b) Figure 3. Optical microscope observations of hair fiber (a) before and (b) after hot-air blow-drying at 70øC for 30 sec. The analysis was performed on the same part of the hair fiber a) b) Figure 4. SEM observations of fiber surface (a) before and (b) after hot-air blow-drying (70øC/30 sec). The analysis was performed on the same part of the hair fiber. of splitting spaces in the cuticle layers through blow-drying is considered to be a more frequent occurrence in daily hair care routines. GENERATION MECHANISMS OF LIGHT SCATTERING IN THE HAIR CUTICLE Figure 6 shows the FT-IR/ATR spectra of hair fibers after application of different blow-drying cycles. The ATR technique provides surface-specific spectra (within 1 micrometer from the surface) with minimal interference from scattering effects, and the measurement depth is therefore within the thickness of the cuticle tissue. The amide I (1650 cm -•) and amide II (1540 cm -•) were the most prominent features of the spectra. The peak observed in the absorbance at -3250 cm-• showed an increase as a function of the number of cycles. This peak is considered to be the peak of an OH group in water molecules that are contained not only in hair fibers, but also in the ambient air. All of the measurements were performed in the dried ambient air, but it was very difficult to control the moisture in the analysis chamber, and, therefore, the peak intensity varied contingently. It is well known that the amide I and II peaks reflect the secondary structure of keratin (9), but no significant changes in these peaks were observed.

358 JOURNAL OF COSMETIC SCIENCE b) Cuticle cell a) Cuticle cell • W• , 5 tam .3/• m e•3•. •e.e•.=,• .•.:•ff•r• -."•" •"•.•:•,•..• •.'•:•. •k•. ' Figure 5. SEM observations of hair fibers for (a) cross and (b) longitudinal sections after hot air blow-drying (70øC/30 sec). .009 ( a) Control 008 b) 5 cycles .................................... c) 30 cycles Amide I •i Amide II .007 - I ..' ? \'.,., ß 3•bo 3oho 2•bo 2oho •bo •obo Wave number / cm 4 Figure 6. FT-IR spectra of hair cuticle (a) before, (b) after five thermal cycles, and (c) after 30 thermal cycles. Each cycle consisted of immersion into de-ionized water followed by 30 seconds of blow-drying at 70øC. We have explored the effect of abrupt changes of moisture content in the hair fiber. Figure 7 shows the relationship between the initial moisture content of the hair fiber and the light-scattering level from glittering speckles on the hair surface just after blow- drying. The amount of glittering speckles was found to be dependent on the initial moisture content. The more the hair fiber is moisturized, the more glittering speckles are observed.