JOURNAL OF COSMETIC SCIENCE 70 C. acnes were identifi ed as 18.01 and 6.12 mM, respectively (Table I). The inhibitory effect of ALACELL against C. acnes was higher than that of 5-ALA. However, antimicrobial activ- ity against S. aureus, B. cereus, E. coli, and Y. enterocolitica did not appear (data not shown). ALACELL DECREASED MELANIN FORMATION WITHOUT CYTOTOXI CITY IN B16F10 CELLS The effect of 5-ALA and ALACELL on B16F10 melanoma c ell proliferation showed that both compounds did not have signifi cant cytotoxicity against the concentration test Figure 1. Chemical structure of ALACELL (A) and analysis by MALDI-TOF mass spectrometry (B). ALACELL was synthesized by combining 5-ALA with Y-G-G-F-L peptide. After purifying by reverse-phase HPLC of ALACELL, a MALDI-TOF mass spectrometry was performed and MALDI-MS data were analyzed by u sing AximaCFR™ Plus-mass spectrometer in positive ion refl ectron mode.

WHITENING AND PROTECTIVE EFFECT OF ALACELL 71 (Figure 2A and B). To study the effect of ALACELL on melanin formation, the melanin formation of 10, 20, and 40 μM on ALACELL-treated B16F10 melanoma cells was quan- tifi ed. Treatment of α-MSH (100 nM) induced a signifi cantly increase of melanin forma- tion (137.2%, p 0.001 Figure 2C). Arbutin (40 μM) and PTU (75 μM) signifi cantly reduced melanin formation in cells induced by α-MSH (Figure 2C p 0.01 and p 0.001, respectively). Five-ALA and ALACELL decreased melanin formation in α-MSH– induced cells, dose dependently (Figure 2C). Among the two compounds, ALACELL decreased more melanin formation at 10, 20, and 40 μM than 5-ALA, respectively. These results indicate that ALACELL exhibits antimelanogenic effi cacy in B16F10 melanoma cells without cytotoxic effect. Figure 2. The cytotoxicity by treatment of ALACELL (A and B), inhibitory effect of ALACELL on melanin formation (C), and inhibitory effect of ALACELL on tyrosinase activity in B16F10 cells (D). B16F10 cell s were treated with concentrations of 10, 20, and 40 μM. After incubation of 48 h, the cells were photographed under a microscope of 2.5 × 104 magnifi cations, and cytotoxicity was measured by the MTT assay. Data are displayed with mean ± standard deviation (n = 3). Signifi cance was indicated at *p 0.05, **p 0.01, and ***p 0.001. Table I The IC50 o f 5-ALA and ALACELL in C. acnes Concentration 5-ALA ALACELL IC50 (mM) 18.01 ± 0.3 6.17 ± .4.0