26 JOURNAL OF COSMETIC SCIENCE AUTHOR CONTRIBUTIONS M.A.: laboratory studies and writing of the article N.Y.: laboratory studies B.M.Y.: statistical analysis. FUNDING SOURCE This study was supported by the Canakkale Onsekiz Mart University Scientific Research Projects Unit (Project ID: FBA-2020-3412). REFERENCES (1) G. M. White, Recent findings in the epidemiologic evidence, classification, and subtypes of acne vulgaris, J. Am. Acad. Dermatol., 39, 34–37 (1998). (2) T. Vos, A. D. Flaxman, M. Naghavi, R. Lozano, C. Michaud, M. Ezzati, et al., Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010, Lancet, 380 (9859), 2163–2196 (2012). (3) B. Bergler-Czop, The aetiopathogenesis of acne vulgaris—what’s new?, Int. J. Cosmetic Sci., 36, 187–194 (2014). (4) R. S. Porter and J. L. Kaplan, Eds., The Merck Manual of Diagnosis and Therapy, 19th Ed. (Merck Sharp &Dohme Corp., Whitehouse Station, NJ, 2011). (5) D. Singh, B. Hatwar, and S. Nayak, Herbal plants and Propionibacterium acnes: an overview, IJBR, 2, 486–498 (2011). (6) S. Nishijima, I. Kurokawa, N. Katoh, and K. Watanabe, The bacteriology of acne vulgaris and antimicrobial susceptibility of Propionibacterium acnes and Staphylococcus epidermidis isolated from acne lesions, J. Dermatol., 27, 318–323 (2000). (7) C. R. Drucker, Update on topical antibiotics in dermatology, Dermatol. Ther., 25, 6–11 (2012). (8) K. Chen, T. J. White, M. Juzba, and E. Chang, Oral isotretinoin: an analysis of its utilization in a managed care organization, J. Manag. Care Pharm., 8, 272–277 (2002). (9) S. Enshaieh, A. Jooya, A. H. Siadat, and F. Iraji, The efficacy of 5% topical tea tree oil gel in mild to moderate acne vulgaris: a randomized, double-blind placebo-controlled study, Indian J. Dermatol. Venereol. Leprol., 73, 22–25 (2007). (10) S. Kapoor and S. Saraf, Topical herbal therapies and complementary choice to combat acne, Res. J. Med. Plant., 5, 650–669 (2011). (11) T. H. Oh, S. S. Kin, W. J. Yoon, J. Y. Kim, E. J. Yang, N. H. Lee, and C. G. Hyun, Chemical composition and biological activities of Jeju Thymus quinquecostatus essential oil against Propionibacterium species inducing acne, J. Gen. Appl. Microbiol., 55, 63–68 (2009). (12) J. Viyoch, N. Pisutthanan, A. Faikreua, K. Nupangta, K. Wangtorpol, and J. Ngokkuen, Evaluation of in vitro antimicrobial activity of Thai basil oils and their microemulsion formulas against Propionibacterium acnes, Int. J. Cosmet. Sci., 28, 125–133 (2006). (13) L. Chularojanamontri, P. Tuchinda, K. Kulthanan, and K. Pongparit, Moisturizers for acne: what are their constituents?, J. Clin. Aesthet. Dermatol., 7, 36–44 (2014). (14) E. Alkhawaja, S. Hammadi, M. Abdelmalek, N. Mahasneh, B. Alkhawaja, and S. M. Abdelmalek, Antibiotic resistant Cutibacterium acnes among acne patients in Jordan: a cross sectional study, BMC Dermatol., 20, no. 17 (2020). (15) V. Cirik, E. Efe, The use of complementary and alternative medicine in children, J. Fam. Med. Community Health, 2, 1031–1034 (2015). (16) K. J. Kemper, S. Vohra, and R. Walls, The use of complementary and alternative medicine in pediatrics, Pediatrics, 122, 1374–1386 (2008).

27 CHARACTERIZATION AND ACTIVITY OF ESSENTIAL OILS (17) A. R. Koroch, H. Rodolfo Juliani, and J. A. Zygadlo, “Bioactivity of essential oils and their components,” in Flavours and Fragrances, R.G. Berger Ed. (Springer Berlin Heidelberg: Berlin and Heidelberg, 2007), pp. 87–115. (18) W. A. Fisk, H. A. Lev-Tov, and R. K. Sivamani, Botanical and phytochemical therapy of acne: a systematic review, Phytother. Res., 28, 1137–1152 (2014). (19) P. J. Houghton, P. J. Hylands, A. Y. Mensah, A. Hensel, and A. M. Deters, In vitro tests and ethnopharmacological investigations: wound healing as an example, J. Ethnopharmacol., 100, 100–107 (2005). (20) J. Lawless, The Illustrated Encyclopedia of Essential Oils: The Complete Guide to the Use of Oils in Aromatherapy and Herbalism, (Element Books, Rockport, MA, 1995). (21) Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Disc Susceptibility Tests Approved Standard—Eleventh Edition, M02-A11 (Wayne, PA, 2012). (22) S. Singh, S. S. Das, G. Singh, C. Schuff, M. P. de Lampasona, and C. A. N. Catalan. Composition, in vitro antioxidant, and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.), Biomed. Res. Int., 18209 (2014). (23) Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically Approved Standard—Eighth Edition, M07-A8 (Wayne, PA, 2009). (24) J. Gutierrez, C. Barry-Ryan, and P. Bourke, The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients, Int. J. Food Microbiol., 124, 91–97 (2009). (25) T. Shimamura, Y. Sumikura, T. Yamazaki, A. Tada, T. Kashiwagi, H. Ishikawa, T. Matsui, N. Sugimoto, H. Akiyama, and H. Ukeda, Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives—inter-laboratory evaluation study, Anal. Sci., 30, 717–721 (2014). (26) K. Slinkard and V. L. Singleton, Total phenol analyses: automation and comparison with manual methods, AJEV, 28, 49–55 (1977). (27) A. Ben Hsouna, N. Ben Halima, S. Smaoui, and N. Hamdi, Citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative effect against Listeria monocytogenes inoculated in minced beef meat, Lipids Health Dis., 16, 146 (2017). (28) E. Noumi, M. Snoussi, H. Hajlaoui, N. Trabelsi, R. Ksouri, E. Valentin, and A. Bakhrouf, Chemical composition, the antioxidant and antifungal potential of Melaleuca alternifolia (tea tree) and Eucalyptus globulus essential oils against oral Candida species, J. Med. Plant Res., 5, 4147–4415 (2011). (29) L. E. Ferreira, B. I. Benincasa, A. L. Fachin, S. H. T. Contini, S. C. Franca, A. C. S. Chagas, and R. O. Beleboni, Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus, Parasites Vectors, 11(1), 269 (2018). (30) I. Marin, E. Sayas-Barbera, M. Viuda-Martos, C. Navarro, and E. Sendra, Chemical composition, antioxidant and antimicrobial activity of essential oils from organic fennel, parsley, and lavender from Spain, Foods, 5, 18 (2016). (31) A. Luis, A. P. Duarte, L. Pereira, and F. Domingues, Chemical profiling and evaluation of antioxidant and anti-microbial properties of selected commercial essential oils: a comparative study, Medicines, 4, 36 (2017). (32) L. Caputo, F. Nazzaro, L. F. Souza, L. Aliberti, L. De Martino, F. Fratianni, R. Coppola, and V. De Feo, Laurus nobilis: composition of essential oil and its biological activities, Molecules, 22, 930 (2017). (33) G. Goger, N. Karaca, B. B. Altınbasak, B. Demirci, and F. Demirci, In vitro antimicrobial, antioxidant and anti-inflammatory evaluation of Eucalyptus globulus essential oil, Nat. Volatiles Essent. Oils, 7, 1–11 (2020). (34) A. Dehghani-Samani, S. Madreseh-Ghahfarokhi, A. Dehghani-Samani, and Y. Pirali, In-vitro antigiardial activity and GC-MS analysis of Eucalyptus globulus and Zingiber officinalis essential oils against Giardia lamblia cysts in simulated condition to human’s body, Ann. Parasitol., 65(2),129–138 (2019). (35) X. Han, C. Beaumont, and N. Stevens, Chemical composition analysis and in vitro biological activities of ten essential oils in human skin cells, Biochim. Open, 5, 1–7 (2017).