31 J. Cosmet. Sci., 74, 31–43 (January/February 2023) *Address all correspondence to Wisanu Thongchai, wisanuthongchai@psru.ac.th Silver Nanoparticles of Bee Honey: Antioxidant and Antimicrobial Activities WISANU THONGCHAI Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand (W.T.) Accepted for publication May 02, 2023. Synopsis In the present study, silver nanoparticles were synthesized from aqueous silver nitrate solution using bee honey and screening the activity of the synthesized nanoparticles against microorganisms. The antioxidant properties of bee honey silver nanoparticles and serum formulations were investigated. The antioxidant and antibacterial properties of aqueous solution of bee honey have been studied using 2.2-dipheny-1-picrylhydrazyl assay and ferric reducing/antioxidant power assay and an agar well diffusion method, respectively. Bee honey was used in bioreduction of silver nitrate to nanoparticles. Ultraviolet (UV)–visible spectrum of silver nanoparticles was found to be 500 nm, and functional groups from Fourier transform infrared spectroscopy from bee honey such as hydroxyl, carboxyl, carbonyl, and phenol groups were important groups in the bioreduction and incorporation of silver ions into nanoparticles. The particle size analysis of the silver nanoparticles was 273 nm in size. The formulated serums were stable and cosmetically appealing with satisfactory pH and viscosity. The bee honey silver nanoparticles and their serum formulation exhibited strong antioxidant and antibacterial activities. The bee honey silver nanoparticles were exhibited with strong antioxidant and antibacterial activities compared to bee honey. In this study, it can be concluded that the serum formulation containing 2% bee honey silver nanoparticles is stable with preferred homogeneity and pH value. INTRODUCTION Bee honey is a natural sweetener liquid that is widely used in various applications where it contains nearly 200 chemical compounds that include carbohydrates such as fructose and glucose (1). Bee honey is a supersaturated sugar solution that contains minerals, proteins, amino acids, vitamins, and nutrients. Part of the dry matter in bee honey contains sugar, which is responsible for its natural composition, viscosity, hygroscopicity, and energy (2). However, the content of bee honey varies depending on the type of plant and the nectar eaten by the bee. Scientific reports have shown that bee honey exhibits important biochemical functions, such as alleviating rhinitis, tonsillitis, and strengthening the immune system (3). Many researchers have confirmed that bee honey is useful in the treatment of wounds, burns, ulcers, and as a healing agent (4). Numerous studies have shown the superiority