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J. Soc. Cosmetic Chemists, 18, 769-775 (Dec. 9, 1967) Hand Degerming Evaluation Utilizing A Split-Use Method JAMES BROWN, JR., M.S., RONALD .J. ERIKSSON, B.S., FRANK YACKOVICH, Ph.D., and DAVID TABER, Ph.D.* Presented November $0, 1066, New York City Synopsis--Utilizing a modified split-use method, two baeteriostatie soaps were evaluated for their utility in degerming the hands. One soap contained 0.75% (by weight) of hexaehloro- phene and 0.75• of 3,4,4'-triehloroearbanilide the other consisted of equal parts by weight of 3,5-di- and 3,4', 5-tribromosalieylanilides, 4,4'-diehloro-3- (trifiuoromethyl)earbanilide, and 3,4,4'-triehloroearbanilide for a total concentration of 2%. A significant reduction in bac- terial counts was achieved by both soaps as compared to a nonmedicated soap. It is empha- sized that rigorous handling of data requires that the confidence interval be identified xvhen per cent reduction is used as a basis for describing degerming efficiency. INTRODUCTION To investigators in the field of soap germicides, many techniques are available for evaluating skin degerming after use of an antibacterial soap. Among these are clinical observations, tape strippings, swab methods, contact plate methods, and plate counts obtained from hand- washings. Four of these methods involve counting bacterial colonies, while clinical trials involve observations of the progress of bacterial dis- eases of the skin. The moderation or the prevention of skin diseases is one of the at- tributes of an effective bacteriostatic soap. However, for the initial evaluation of a new bacteriostatic system in soap, the length of time needed for a meaningful clinical study makes this tool of questionable value. * Armour Grocery Products Company, 3115 S. Benson St., Chicago, Ill. 60609. 769