77O JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS (111) 112) 113) (114) (115) (116) (117) (118) (119) (•20) (121) (122) (123) (124) (125) (126) (127) (128) (129) (130) (131) (132) (133) (•34) (135) (136) (137) (138) (139) 1.140) Reid, J. and Jarrett, A. Enzymatic and histological effects of a standard stimulus. Arch. Dermatol. 95 632 (1967). Lansdown, A. B. G. and Grasso, P. (unpublished observations). Szakall, A. Biologie der Hautoberflaeche. Proc. 1 lth Int. Congr. Dermatol. Stockholm. II 23 (1957). Blank, I. H. Factors which influence the water content of the stratum corneum. J. Invest. Dermatol. 18 433 (1952). Szakall, A. Ueber die Eigenschaften, Herkunft und physiologischen Funktionen der H-Ionkonzentration bestimmenden Wirkstoffe in der verhornten Epidermis. Arch. Klin. Exp. Dermatol. 201 331 (1955). Spier, H. W. and Pascher, G. Analytische und physiologische Untersuchungen ueber die wasserloeslichen Inhaltsstoffe der peripheren Hornschicht (Hautoberflaeche). Proc .11th Int. Congr. Derm. Stockholm. II, 14 (1957). Middleton, J. D. Mechanism of water binding in stratum corneum. Brit. J. Dermatol. 80 437 (1968). Jacobi, O. Water and water vapour absorption of the stratum comeurn of the living human skin. J. Appl. Physiol. 12 403 (1968). Van Scott, E. J. and Lyon, J. B. A chemical measure of the effect of soap and detergents on the skin. J. Invest. Dermatol. 21 199 (1953). Harrold, S. P. Denaturation of epidermal keratin by surface active agents. J. Invest. Dermatol. 32 581 (1959). Flesch, P. and Kun, E. A colorimetric method for determination of sulphydryl groups in tissue homogenates by 1-(4-chloromercuriazo)-naphthol-2. Proc. Soc. Exp. Biol. Med. 74 249 (1950). Smeenk, G. The influence of detergents on the skin (a clinical and biochemical study). Arch. Klin. Exp. Dermatol. 235 180 (1969). Vermeer, D. J. H., de Jong, J. C., Donk, L. and Leemhuis, J. Een onderzoek naar de werking van wasmiddelen op de huid. Ned. Tijdschr. Geneesk. 107 1768 (1963). Nicholaides, N., Fu, H. C. and Rice, G. R. The skin surface lipids of man compared with those of eighteen species of animals. J. Invest. Dermatol. 51 83 (1968). Downing, D. T., Strauss, J. S. and Pochi, P. E. Variability in the chelrfical composition of human skin surface lipids. J. Invest. Dermatol. 53 (1969). Downing, D. T. Lipolysis by human skin surface debris in organic solvents. J. Invest. Dermatol. 54 395 (1970). Blank, I. H. Further factors which influence the water content of the stratum corneum. J. Invest. Dermatol. 21 259 (1953). Grasso, P. and Lansdown, A. B. G. Methods of measuring and factors affecting per- cutaneous absorption. J. Soc. Cosmet. Chem. 23 481 (1972). Wolrab, W. and Gruneberg, J. Histochemische Befunde an der klinisch gesunden Haut von Psoriatikern. Arch. Klin. Exp. Dermatol. 225 259 (1966). Adams, P. D. The effect of age on consumption of oxygen and composition of the skin of the albino rat. Arch. Dermatol. Syph. 36 606 (1936). Cook, E. S., Hart, M. J. and Joly, R. A. The effect of 1,2,5,6-dibenzanthracene on the growth and respiration of yeast. Science 87 331 (1938). Bronfinbrermer, J., Hershey, A.D. and Doubly, J. A. Evaluation of germicides by a manometric method. Proc. Soc. Exp. Biol. Med. 38 210 (1938). Amersbach, J. C., Nutini, L. G. and Cook, E. S. Skin respiration as a dermatologic tool. Arch. Dermatol. Syph. 43 949 (1941). Naylor, P. F. D. and Evans, N. T. S. Oxygen transport through the skin. Brit. J. Dermatol. 81 62 (1969). Tarantino, C. Oxygen consumption of tissues as a function of hydrogen ion concentration of the media. Riv. Biol. 23 281 (1937). Wohlgemuth, J. and Klopstock, F. Die Fermente der Haut. V Atmung und Glycolyse der Haut und ihre Beeinflussung durch Hormone. Blochem. Z. 175 202 (1926). Brown, V. K. H. The influence of some detergents on the uptake of oxygen by 'hairless' mice. J. Soc. Cosmet. Chem. 20 413 (1969). Barron, E. S. G., Meyer, J. and Miller, Z. B. The metabolism of the skin. The effect of vesicant agents. J. Invest. Dermatol. 11 97 (1948). Leibshon, E., Appel, B., Ullrick, W. C. and Tye, M. J. Respiration of human skin. Normal values, pathological changes and effect of certain agents on oxygen uptake. J. Invest. Dermatol. 30 1 (1958). Cruickshank, C. N. D., Trotter, M. D and Cooper, J R. A study of available substrates for endogenous respiration of skin in vitro. J. Invest. Dermatol. 39 175 (1962).

APPRAISAL OF METHODS FOR DETECTING PRIMARY SKIN IRRITANTS 771 (141) (142) (143) (144) (145) (146) (147) (148) (149) 050) (151) (152) (153) (154) 055) (156) (157) 058) 059) (160) (161) (162) (163) (164) (165) (166) (167) (168) (169) (170) (171) Dushoff, I. M., Payne, J., Hershey, F. B. and Donaldson, R. C. Oxygen uptake and tetrazolium reduction during skin cycle of the mouse. Amer. J. PhysioL 209 231 (1965). Mezei, M., Sager, R. W., Stewart, W. D. and de Ruyter, A. L. Dermatitic effect of non- ionic surfactants. I. Gross, microscopic and metabolic changes in rabbit skin treated with non-ionic surface active agents. J. Pharm. Sci. 55 584 (1966). Harmher, H. and Hellerstrom, C. The oxygen consumption of the germinal epithelium in normal and psoriatic skin. Brit. J. Dermatol. 83 371 (1970). Keilin, D. and Hartree, E. F. The activity of the succinic dehydrogenase-cytochrome system in different tissue preparations. Biochem. J. 44 205 (1949). Verbov, J. Hypohidrotic (or anhidrotic) ectodermal dysplasia--an appraisal of diagnostic methods. Brit. J. Dermatol. 83 341 (1970). Wada, M and Takagaki, T. A simple and accurate method for detecting the secretion of sweat. Tohoku J. Exp. Med. 49 284 (1948). Juhlin, L. and Shelley, W. B. A stain for sweat pores. Nature (London) 213 408 (1967). Sarkany, I. A method for studying the microtopography of the skin. Brit. J. Dermatol. 74 254 (1962). Tronnier, H. and Eisbacher, I. Ueber eine neueMethode zurMessung der Rauhigkeit der Haut. Berufsdermatosen 18 89 (1970). Wheatley, V. R and James, A. T. Studies of sebum. 7. The composition of the sebum of some common rodents. Biochem. J. 65 36 (1957). MacKenna, R. M. B., WheatIcy, V. R. and Wormall, A. Composition of the surface skin fat (sebum) from human forearm. J. Invest. Dermatol. 15 33 (1950). Roeth, K. The sebographical akin test. In Middleton, A. W. (ed) Cosmetic Science 1962, 21 (1963) {Pergamon Press, Oxford). Hjorth, N. and Fregert, S. in Rooke, A., Wilkinson, D. S., Ebling, F. J. G. Contact dermatitis in Textbook of dermatology (1968) (Blackwell Scientific Publications, Oxford). Draize, J. H. The determination of the pH of the skin of man and common laboratory animals. J. Invest. Dermatol. 5 77 (1942). Marchionini, A. and Schade, H. Der Saeuremantelder Haut (nach Gaskettenmessungen). Klin. Wochenschr. 7 12 (1928). Marples, B. J. in Chas. C. Thomas. The ecology of the human akin (1965) (Springfield, Illinois). Vesey, C. M. R. and Calnan, C. D. Alkali neutralization of the skin. Trans. Rep. St John's Hosp. Dermatol. Soc. 41 38 (1958). Stupel, H. and Szakall, A. in Dr. A. Huthing. Die Wirkung von Waschmitteln auf die Haut (1957) (Heidelberg). Rothman, S. Physiology and biochemistry of the akin (1954) (University of Chicago Press). Schultz K. H. in Gottron, H. A. and Shonfeld, W. Berufsdermatosen. Dermatologie und Venereology V 574 (1963) (George Thieme Verlag, Stuttgart). Spier, H. W. Die Bedeutung der wasserloeslichen Inhaltsstoffe im Rahmen der Schutzfunk- tionen des Stratum Corneum 1 42 (1962) (Symposium Dermatologoram, Prague). Heuss, E. Die Reaktion des Schweiss beim gesunden Menschen. Montsh. prakt. Dermatol. 14 343 (1892). Sharlit, H. & Scheer, M. The hydrogen ion concentration of the surface of the healthy intact skin. Arch. Dermatol. Syph. 7 592 (1923) Levin, O. L. and Silvers, S. H. The reaction of the skin and its secretions in eczema. I. The hydrogen ion concentration of the skin surface in eczema. Arch. Dermatol. Syph. 25 825 (1932). Blank, I. H. Measurement of the pH of the skin surface. I. Technique. J. Invest. Dermatol. 2 67 (1939). Saitoh, M. and Inomato, M. Dermal neutralizing ability. In Middleton, A.W. (ed) Cosmetic Science 1962, 93 (1963) (Pergamon Press, Oxford). Tregear, R. T. in Physical functions of skin (1966) Theoretical and Experimental Biology, 5 (Academic Press, London & New York). Tregear, R. J. in Rook, A. and Champion, R. H. The permeability of skin to molecules of widely differing properties. Progress in the Biological Sciences in Relation to Dermato- logy H (1964) (Cambridge University Press, Cambridge). Wenzel, H. G. Study of the tearing and stretching of skin. Zentbl. Allg. Pathol. Pathol. Anat. 85 117 (1948). Dick, J. C. The tension and resistance to stretching of human skin and other membranes, with results from a series of normal and oedematous cases. J. Physiol. 112 102 (1951). Brown, L., Harkhess, M. L. R. and Harkness, R. D. Effect of hydrazine and other aide- hyde reacting agents on mechanical properties of rat tail skin. Acta Phys. Acad. Sci. Httng. 36 157 (1969).