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J. Soc. Cosmet. Chem. 24 685-692 (1973) ¸ 1973 Society of Cosmetic Chemists of Great Britain Eye irritation: studies relating to responses in man and laboratory animals N.J. VAN ABBI•* Synopsis---A male HAIRDRESSING formulated as a solid transparent MICRO-EMULSION gave essentially negative responses in EYE IRRITANCY screening tests conducted in RABBITS and squirrel MONKEYS. Nevertheless, soon after introduction into a test-market, complaints of human EYE IRRITATION arose. Visual blurring was a distressing though reversible symptom. The adverse effects invariably followed exposure to rain or snow. Somewhat similar responses in human volunteers were noted with an aqueous solution of the ETHOXYLATED OLEYL ALCOHOL incorporated in the formulation but were diminished by changing to a less hydrophilic ethoxylate. Transient corneal pitting seemed to be a relevant observation in laboratory animal eye tests in relation to the visual blurring which occurred in man. Tests for chemically-induced eye irritancy are carried out using laboratory animals in the hope of providing a reliable basis for predicting responses in the human eye. This report, however, is concerned with circum- stances in which essentially negative findings were obtained in laboratory screening tests although relatively severe visual disturbances and discomfort occurred soon after the product was launched in a test-market. The product was a male hairdressing which had the appearance of a transparent light blue-coloured gel the formulation was an oil-in-water emulsion, the disperse phase being in the form of extremely fine globules (probably 10-60 nm in diameter (1)). The formula was: * Beecham Products, Applied Research & Evaluation Unit, RandalIs Rd, Leatherhead, Surrey. 685