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j. Soc. Cosmet. Chem., 31,219-222 (July/August 1980) Nitrosamine determination by use of conventional equipment VICTOR H. BAPTIST, Ph.D. and RICHARD BROWN, B.S., Max Factor & Co. 1655 N. Mc Cadden Place, Hollywood, CA 90028. Received January 11, 1980. Presented at the Annual Scientific Meeting, Society of Cosmetic Chemists, December 6-7, 1979, New York, New York. Synopsis The determination of NITROSAMINES in consumer products has heretofore been hampered by the need for costly specialized equipment. An effective technique to analyze for total nonvolatile nitrosamines has been developed which utilizes only conventional equipment. The method is as specific as the thermal energy analyzer. We are able to achieve a sensitivity of better than one part per billion. Six to seven analyses can be completed each day by one technician. In addition, the technique is also adaptable to determining if NITRITES are present in raw materials. We have found the method successful in use on cosmetic products, grilled hot dogs, beer and cutting fluids. INTRODUCTION Nitrosamines have attracted an increasing amount of attention of cosmetic manufac- turers due to the disclosure by Fine in 1977 of their presence in cosmetics. The thermal energy analyzer has been the method of specific nitrosamine detection due to its ability of detecting nitrosamines at parts per billion. Industry needed an equally sensitive method that was less costly. The colorimetric technique that follows which detects total nitrosamines met these goals. One technician can complete 6 to 7 samples per day. In addition the technique is also adaptable to determine "nitrite" present in raw materials. EXPERIMENTAL APPARATUS AND REAGENTS All work was performed utilizing a Beckman DGB or Hitachi 100-60 spectrophotom- eter, vacuum oven, vacuum pump, ultrasonic cleanser, 4 oz. glass bottles with teflon liners, and 8 ml glass vials plus standard chemical glassware. Standard N-nitrosodiethanolamine (NDE1A) was obtained from Columbia Organic Chemical Co. (Columbia, South Carolina) 1-naphthylamine-7-sulfonic acid from Pfaltz 219