270 JOURNAL OF THE SOCIETY OF COSMETIC CHEMISTS The concentration levels of permeated mercaptans into the glove portion were assayed by using the external standard quantitation technique from calibration curves Fig. 1 and Fig. 2. Table II and Table III list the concentration levels of GMTG and AMTG found into the interior portion of glove type at different time intervals. PERMEATION STUDIES Two sets of experimentation were devised for determining the amount of permeation through the gloves. Method A. A pressure gradient of 1:10 water to GMTG solution composed of 19% glyceryl monothioglycolate based on an 80% active composition in anhydrous glycerin was dissolved in an aqueous pH 8 solution buffered with ammonium carbonate. The middle finger portion of the glove was excised and filled with aleionized water (10 ml), followed by knotting the open end. The mixture was placed into a 200-ml beaker and stirred with a magnetic stirrer capped with a glass cover. The mixture was stirred for a given time interval and then the glove portion removed long enough to rinse the exte- rior with aleionized water. The water inside the glove portion was removed with a glass pipette and the contents put into a glass vial. A 200-•1 portion was analyzed by HPLC. One milliliter of the remaining portion was mixed with 5 ml of the DNTB reagent and the mixture analyzed by u.v. method 1 but using 410-nm detection. Table II HPLC Direct & DTNB Indirect Analysis Data of Glycerylmonothioglycolate (GMTG) and Ammonium Thioglycolate (AMTG) Time Glove 0 min 10 min 30 min 60 min Final PN ppm GMTG (HPLC direct)/ppm GMTG (DNTB indirect) A 0/0 0/0 0/0 0/0 24 hrs 31/ B 0/0 0/0 0/0 0/0 C 0/0 0/0 0/0 0/0 D 0/0 0/0 0/0 0/0 2.5 hrs / 2 E 0/0 0/0 0/0 0/0 2.5 hrs /4.15 F 0/0 0/0 0/0 0/0 17 hrs 60/ G 0/0 0/0 0/0 0/0 21 hrs 0/0 H 0/0 0/0 0/0 0/0 21 hrs 0/0 I 0/0 0/0 0/0 0/0 43 hrs 0/0 ppm AMTG (HPLC direct)/ppm AMTG (DNTB indirect) A 0/0 0/0 0/0 0/0 24 hrs 5/ B 0/0 0/0 0/0 0/0 17 hrs 0/0 C 0/0 0/0 0/0 0/0 17 hrs 5/ D 0/0 0/0 0/0 0/0 19 hrs 18/ E 0/0 0/0 0/0 0/0 F 0/0 0/0 0/0 0/0 17 hrs 5/ G 0/0 0/0 0/0 0/0 21 hrs 0/0 H 0/0 0/0 0/0 0/0 I 0/0 0/0 0/0 0/0 PN = Permeation noted

GLOVES FOR USE WITH COSMETICS 271 Table IlI Analysis of GMTG and AMTG in Stretched Gloves Glove 0 min 30 min 60 min 150 min HPLC direct ppm GMTG/DNTB indirect ppm GMTG A 0/0 B 0/0 C 0/0 D 0/0 E 0/0 F 0/0 G 0/0 H 0/0 I 0/0 o/o o/o o/o o/o o/o o/o o/2 o/o HPLC direct ppm AMTG/DNTB indirect ppm AMTG 0/4.15 0/0 0/0 A 0/0 B 0/0 C 0/0 D 0/0 E 0/0 F 0/0 G 0/0 H 0/0 I 0/0 0/0 o/o o/o o/o o/o o/o o/o o/o Method B. The middle finger portion of each type of glove was stretched about the open end ofa 15-mm by 175-mm test tube. The exposed glove area was 2.54 cm 2. The glove portion was filled with 10 ml of test solution and the glove secured against leakage by knotting it securely. This experiment series was performed to mimic the expansion a glove material undergoes upon flexation of the hand. Ideally, any open channels within the structure of the glove would be witnessed by the detection of an increased amount of the mercaptan. The stretched glove portion was then placed into a 200-ml beaker equipped with a magnetic spin bar and filled with 100 ml of either the GMTG or AMTG solution detailed above. At timed intervals the stretched glove apparatus was removed from the beaker, rinsed with water, and the contents analyzed by HPLC directly and via the DNTB enhancement technique. Hydrogen peroxide permeation test method. Portions of the tested gloves were filled with 10 ml of deionized water, the system knotted at its ends, placed in a 200-ml beaker capped with a glass cover plate, and stirred for a timed interval with a magnetic stirrer in 2.5% hydrogen peroxide buffered to pH 3.2 with phosphate/phosphoric acid (0.05 M). The glove was removed, rinsed with deionized water, and the interior contents poured into a 10-ml volumetric flask. Next the contents were examined for peroxide content using the Merckquant peroxide test based on peroxidase conversion of hydrogen peroxide. The combination of peroxide was determined by comparing the color of the test strip with the indicator chart of the test kit.